1. 1 CELLULAR RESPIRATION: HARVESTING CHEMICAL ENERGY Section A: The Principles of Energy Harvest

2. 2 مـراحـل الـتـنـفـــس الــدم حويصلات هوائية خلايا الجـســ م 1- تـنـفـس خـارجـي 2- تـنـفـس داخـلــي 3- تـنـفـس خـلـوي

3. Objectives The Principles of Energy HarvestThe Principles of Energy Harvest Cells recycle the ATP.Cells recycle the ATP. Redox reactions release energy.Redox reactions release energy. The “fall” of electrons during respirationThe “fall” of electrons during respiration

4. Overall process a)Organic compounds + O 2 → CO 2 + H 2 O + energy b)B. Food is the fuel for cellular respiration c)C. Cellular respiration is a catabolic pathway: it releases energy by breaking down complex molecules) d)D. Cellular respiration involves movement of electrons (gain or loss) e)E. We will study the breakdown of glucose as an example

5. Section A: The Principles of Energy Harvest 1.Cellular respiration and fermentation are catabolic, energy-yielding pathways 1.Cellular respiration and fermentation التخمر are catabolic, energy-yielding مُنتِج للطاقة هدْم pathways 2.Cells recycle the ATP they use for work 3.Redox reactions release energy when electrons move closer to electronegative atoms 3.Redox reactions تفاعلات الأكسدة-الإختزال release energy when electrons move closer to electronegative atoms 4.Electrons “fall” from organic molecules to oxygen during cellular respiration 4.Electrons “fall” تنتقل from organic molecules to oxygen during cellular respiration 5. The “fall” of electrons during respiration is stepwise, via NAD + and an Electron Transport Chain 5. The “fall” of electrons during respiration is stepwise مَرْحَلي, via NAD + and an Electron Transport Chain

6. Organic molecules store energy in their arrangement of atoms.Organic molecules store energy in their arrangement of atoms. Enzymes catalyze the systematic degradation of organic molecules that are rich in energy to simpler products with less energy.Enzymes catalyze the systematic degradation of organic molecules that are rich in energy to simpler products with less energy. Some of the released energy is used to do work and the rest is dissipated as heat.Some of the released energy is used to do work and the rest is dissipated as heat. Metabolic pathways that release the energy stored in complex organic molecules are catabolic.Metabolic pathways that release the energy stored in complex organic molecules are catabolic هدْمي. Fermentation is a type of catabolic process leads to the partial degradation of sugars in the absence of oxygen.Fermentation is a type of catabolic process leads to the partial degradation التحلل الجزئي of sugars in the absence of oxygen. Cellular respiration is a more important catabolic process, uses oxygen as a reactant to complete the breakdown of a variety of organic molecules.Cellular respiration is a more important catabolic process, uses oxygen as a reactant to complete the breakdown of a variety of organic molecules. This process is:This process is: Organic compounds + O 2 -> CO 2 + H 2 O + EnergyOrganic compounds + O 2 -> CO 2 + H 2 O + Energy Carbohydrates, fats, and proteins can all be used as the fuel, but we will start learning with glucose.Carbohydrates, fats, and proteins can all be used as the fuel, but we will start learning with glucose. C 6 H 12 O 6 + 6O 2 -> 6CO 2 + 6H 2 O + Energy (ATP + heat)C 6 H 12 O 6 + 6O 2 -> 6CO 2 + 6H 2 O + Energy (ATP + heat) 1. Cellular respiration and fermentation are catabolic, energy-yielding pathways 1. Cellular respiration and fermentation are catabolic, energy-yielding مُنتج للطاقة pathways

7. Food (Fuel of energy)Food RespirationRespiration O2O2 O2O2EnergyEnergy H2OH2OH2OH2O CO 2 + Cellular Activities Cellular Respiration Organic compounds + O 2 Energy + CO 2 + H 2 O

8. ATP (Adenosine Tri-Phosphate) is the important molecule in cellular energetics.ATP (Adenosine Tri-Phosphate) is the important molecule in cellular energetics عمليات إنتاج الطاقة. –The attachment of three negatively-charged phosphate groups (P) is an unstable, energy-storing arrangement. –The attachment of three negatively-charged phosphate groups (P) is an unstable عير مستقر, energy-storing مخزن للطاقة arrangement. –Loss of the end phosphate group release energy. –Thus, it can diffuse to any part of the cell and release energy The price of most cellular work is the conversion of ATP to ADP and phosphate (P).The price of most cellular work is the conversion of ATP to ADP and phosphate (P). An animal cell regenerates ATP from ADP by adding P via the catabolism of organic molecules.An animal cell regenerates تعيد إنتاج ATP from ADP by adding P via the catabolism هدم of organic molecules. 2. Cells recycle the ATP they use for work

9. Adenosine Tri-Phosphate (ATP) PPP P PPPP + H 2 O EnergyEnergy Adenosine Triphosphate Adenosine Di-Phosphate

10. The transfer of the terminal phosphate group from ATP to another molecule is phosphorylation. The transfer of the terminal phosphate group from ATP to another molecule is phosphorylation فـَسْـفـَرة. This changes the shape of the receiving molecule in order to work (transport, mechanical, or chemical). When the phosphate groups leaves the molecule, the molecule returns to its original shape (stop).

11. How dose ATP drive cellular work ? MicrotubuleMicrotubule Motor Protein Organelle P Energy P PPPP Cell respiration P

12. Catabolic pathways relocate يبدل أماكن the electrons stored in food molecules, releasing energy that is used to synthesize لتخليق ATP.Catabolic pathways relocate يبدل أماكن the electrons stored in food molecules, releasing energy that is used to synthesize لتخليق ATP. Oxidation-reduction reactions (Redox reactions):Oxidation-reduction reactions (Redox reactions): Are reactions that result in the transfer of one or more electrons from one reactant to another Are reactions that result in the transfer of one or more electrons from one reactant to another Oxidation: Is the loss of electrons.Oxidation: Is the loss فقـد of electrons. Reduction: Is the addition of electrons.Reduction: Is the addition إكتساب of electrons. 3. Redox reactions release energy when electrons move closer to electronegative atoms e-e- XYXY ++ + Oxidation ( reducing agent ) Reduction ( oxidizing agent )Energy Na + Cl Na + + Cl - Redox reactions require both a donor and acceptor of e. both a donor and acceptor of e.Oxidation (Reducing agent) Reduction (Oxidizing agent) Lose electrons Gain electrons Lose hydrogen Gain hydrogen Gain oxygen Lose oxygen

13. In cellular respiration, glucose and other fuel molecules are oxidized, releasing energy.In cellular respiration, glucose and other fuel molecules are oxidized, releasing energy. Glucose is oxidized, oxygen is reduced, and electrons loose potential energy.Glucose is oxidized, oxygen is reduced, and electrons loose potential energy. H is the source of electrons that transfere to O.H is the source of electrons that transfere to O. Thus, molecules that have an abundance of hydrogen are excellent fuels because their bonds are a source of electrons that “fall” closer to oxygen.Thus, molecules that have an abundance of وفرة من hydrogen are excellent fuels because their bonds are a source of electrons that “fall” closer to oxygen. Enzymes lower the barrier of activation energy, allowing these fuels to be oxidized slowly.Enzymes lower the barrier of activation energy, allowing these fuels to be oxidized slowly. When H moves to O, it leaves bonds which degenerated to release energy.When H moves to O, it leaves bonds which degenerated to release energy. The resulting energy is used by the cell to synthesis ATP.The resulting energy is used by the cell to synthesis ATP. 4. Electrons “fall” from organic molecules to oxygen during cellular respiration C 6 H 12 O 6 + 6O 2 6CO 2 + 6H 2 O + (ATP + Heat) Energy = 686 kcal/mol e-e- Oxidizing agent Reducing agent Energy

14. Cellular respiration does not oxidize glucose in a single step that transfers all the hydrogen in glucose to oxygen at one time.Cellular respiration does not oxidize glucose in a single step that transfers all the hydrogen in glucose to oxygen at one time. Rather, glucose and other fuels are broken down gradually in a series of steps, each catalyzed by a specific enzyme.Rather, glucose and other fuels are broken down gradually تدريجيا in a series of steps, each catalyzed by a specific enzyme. At key steps, hydrogen atoms move from glucose and passed first to the coenzyme NAD + (Nicotinamide Adenine Dinucleotide).At key steps فى الخطوات الأساسية, hydrogen atoms move from glucose and passed first to the coenzyme NAD + (Nicotinamide Adenine Dinucleotide). Dehydrogenase enzymes strip two hydrogen atoms from the fuel (e.g., glucose), pass two electrons to NAD + and release H +.Dehydrogenase enzymes strip two hydrogen atoms from the fuel (e.g., glucose), pass two electrons to NAD + and release H +. This changes the oxidized form, NAD+, to the reduced form NADH. Thus, NAD+ is oxidizing agent as it accept electrons.This changes the oxidized form, NAD+, to the reduced form NADH. Thus, NAD+ is oxidizing agent as it accept electrons. – NAD + functions as the oxidizing agent in many of the redox steps during the catabolism of glucose. As electrons “fall” from NADH to oxygen, their energy is used to synthesize ATP. 5. The “fall” of electrons during respiration is stepwise, by NAD + and an electron transport chain 5. The “fall” of electrons الإنحدار الإليكتروني during respiration is stepwise مرحلي, by NAD + and an electron transport chain H-C-OH + NAD + C=O + NADH + H + Dehydrogenase

15. The most common carrier is NAD +The most common carrier is NAD + H atoms have one proton and one electronH atoms have one proton and one electron When two H atoms are removed from a substrate NAD + accepts the electrons from both atoms and a proton from one of themWhen two H atoms are removed from a substrate NAD + accepts the electrons from both atoms and a proton from one of them NAD + + 2H→ NADH + H +NAD + + 2H→ NADH + H +

16. Cellular respiration uses an electron transport chain to break the fall of electrons to O 2 into several steps.Cellular respiration uses an electron transport chain سلسلة نقل الإليكترونات to break يـُقـَسم the fall of electrons to O 2 into several steps عدة خطوات. The electron transport chain, consisting of several molecules (), is built into the inner membrane of a mitochondrion.The electron transport chain, consisting of several molecules (primarily proteins), is built into the inner membrane of a mitochondrion. NADH takes electrons from food to the “top” of the chain.NADH takes electrons from food to the “top” of the chain. At the “bottom”, oxygen captures the electrons and H + to form water.At the “bottom”, oxygen captures the electrons and H + to form water. The free energy change from “top” to “bottom” is -53 kcal/mole of NADH.The free energy change from “top” to “bottom” is -53 kcal/mole of NADH. Electrons are passed by increasingly electronegative molecules in the chain until they are caught by oxygen (the most electronegative).Electrons are passed by increasingly electronegative molecules in the chain until they are caught by oxygen (the most electronegative).

17. Electron Fall The Cell Summary of electron “Fall” steps during respiration - Falling of all H atoms from glucose to O is gradually not at once. - It occurs in steps, each one is catalyzed by an enzyme. - H atoms of glucose pass first to the co-enzyme NAD + to form NADH - Then from NAD H to electron transport chain, and finally to O and releases energy to form ATP. MitochondrionMitochondrion Food NADH Transport chain Oxygen NAD + Hee ADPATPEnergy