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EE Slawson Tempel, © WUSTL. STOP EE Slawson Tempel, © WUSTL.

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Presentation on theme: "EE Slawson Tempel, © WUSTL. STOP EE Slawson Tempel, © WUSTL."— Presentation transcript:

1 EE Slawson Tempel, © WUSTL

2 STOP EE Slawson Tempel, © WUSTL

3 STOP EE Slawson Tempel, © WUSTL

4 OH STOP EE Slawson Tempel, © WUSTL

5 OH STOP EE Slawson Tempel, © WUSTL

6 STOP EE Slawson Tempel, © WUSTL

7 STOP EE Slawson Tempel, © WUSTL

8 STOP EE Slawson Tempel, © WUSTL

9 STOP EE Slawson Tempel, © WUSTL

10 STOP OH EE Slawson Tempel, © WUSTL

11 OH STOP EE Slawson Tempel, © WUSTL

12 OH STOP EE Slawson Tempel, © WUSTL

13 STOP EE Slawson Tempel, © WUSTL

14 STOP EE Slawson Tempel, © WUSTL

15 STOP EE Slawson Tempel, © WUSTL

16 STOP EE Slawson Tempel, © WUSTL

17 STOP OH EE Slawson Tempel, © WUSTL

18 G…G… © Illumina, EEST, © WUSTL

19 GC…GC…

20 GCT…GCT…

21 GCTG…GCTG…

22 GCTGA…GCTGA…

23 Flowcell 8 lanes For picture taking: Each lane is broken up into 100 tiles, each fluor is imaged separately – 2400 pictures taken per cycle Camera time is the limiting step! EE Slawson Tempel, © WUSTL

24 STOP Chemistry problem 1: terminator is retained EE Slawson Tempel, © WUSTL

25 OH Chemistry problem 2: fluor is retained EE Slawson Tempel, © WUSTL

26 STOP Chemistry problem 2: fluor is retained EE Slawson Tempel, © WUSTL

27 STOP Chemistry problem 2: fluor is retained EE Slawson Tempel, © WUSTL

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