1. *DNA Fingerprinting* Glue notes into pg 51 Glue paternity tests sheet into pg 50

2. Background Info People who have different characteristics also have different DNA sequences People who have different characteristics also have different DNA sequences –Ex. Person #1 ATTCCGAT – Person #2 TCGGCTAT The more variation in features, the more variation in DNA sequences The more variation in features, the more variation in DNA sequences We have learned to identify these sequences We have learned to identify these sequences

3. Uses Diagnosis of inherited disorders in all ages Diagnosis of inherited disorders in all ages Paternity suits Paternity suits Identification of bodies Identification of bodies Crime investigations Crime investigations

4. Method 1. Extract the DNA 1. Extract the DNA –from cells (blood, skin, hair, cheek) –only small amount needed –same pattern found in every cell in an organism 2. Cutting the DNA 2. Cutting the DNA – use restriction enzymes to cut at a specific place –ex. EcoR1 cuts when it see’s GAATTC

6. Cont. 3. Sorting the DNA 3. Sorting the DNA –DNA pieces are sorted electrically according to size using the process of gel electrophoresis 4. Staining 4. Staining –Add radioactive/colored dye so it can be seen

7. DNA Fingerprint Larger pieces Smaller Pieces

8. Crime Scene

9. Paternity Cases Every band the child has must match up with either mom or dad. If they don’t the guy is NOT THE FATHER