1. 1 Advisor : Cheng-Hsin Chuang Advisee :Wan-Ting Su Department of Mechanical Engineering & Institute of Nanotechnology, Southern Taiwan University, Tainan, TAIWAN Date ﹕ 2012/04/06 Paper Survey A microwire sensor for rapid detection of Escherichia coli K-12 in fresh produce Innovate Food Science and Emerging Technologies 12 (2011) 617-622 Sungkyun Kim,Lin Lu, Jae-Hyun Chung,Kyonghoon Lee,Yong Li,Soojin Jun

2. Outline Paper Survey  Sensor Design  Results and discussion Experimental Result Future work

3. Sensor Design Fig.1. ( a) Microwire sensing unit (b) Power supply and measurement equipments

4. Results and Discussion DEP force and electroosmotic flowCapillary Fig2.Regression curve for enumeration of E.coli K-12cells Fig3.Stepwise produres for detection of E.coli K-12

5. Simulation Result Fig 4. Sequential simulation of electric field strength

6. Experiment Results Fig. 5. FESEM image of E.coli K-12 cells captured on the microwire. (a)Without and (b) with AC field ﹒

7. Experiment Results Fig. 6. Optimization of the working frequency for detection of E.coli K-12 at 10 6 CFU/ml Fig.7. Fluorescence intensity of E.coli K-12 in PBS buffer when applied with 20V PP and 3MHz

8. Experiment Results Fig. 8. Selective capture of bactrial cells from E.coli K-12 internalized spinach solution: (a) FI values of E.coli K-12 in spinach and buffer solutions at different concentrations (b) FSEM image of the microwire sensor. Many spinach particles are captured on the wire surface

9. Outline Paper Survey  Sensor Design  Results and discussion Experimental Result Future work

10. Experimental MethodExperimental

11. Experimental Results Flow Rate ： 0.1 ml /hr Al 2 O 3 Concentration：2 wt% BSA Concentration：1 μM Applied Frequency：10 kHz Apply Voltage：10 Vpp Applied Time ： 0 min Applied Time ： 2 min Applied Time ： 16 min Applied Time ： 30 min

12. Outline Paper Survey  Sensor Design  Results and discussion Experimental Result Future work

13. Paper Survey Experiment by Different Parameter Experimental Sample Using Bladder Cancer Cell SEM Training

14. Thank you for your attention !!