1. Glycogen Regulation Pathways Produced by Dr. Steven Pelech Kinexus Bioinformatics Corporation Copyright 2010

2. S645 p38  MAPKPHK  1 PYGM S15 PYGL S15 PYGB S15 GYS1CK1MAPKAPK2DYRK1ADYRK2DYRK1B Glycogen Regulation AMPKa  PKA S11 S641 S645 cAMP S8 GYS2 S641 S8 S82 Glycogen Degradation AMP Ca 2+ Grk3PASK GSK3 

3. S645 p38  MAPKPHK  1 PYGM S15 PYGL S15 PYGB S15 GYS1CK1MAPKAPK2DYRK1ADYRK2DYRK1B Glycogen Regulation AMPKa  PKA S11 S641 S645 cAMP S8 GYS2 S641 S8 S82 Glycogen DegradationGlycogen Synthesis AMP Ca 2+ Grk3PASK GSK3 

4. S645 p38  MAPKPHK  1 PYGM S15 PYGL S15 PYGB S15 GYS1CK1MAPKAPK2DYRK1ADYRK2DYRK1B Glycogen Regulation AMPKa  PKA S11 S641 S645 cAMP S8 GYS2 S641 S8 S82 Glycogen DegradationGlycogen Synthesis AMP Ca 2+ Grk3PASK GSK3 

5. S645 p38  MAPKPHK  1 PYGM S15 PYGL S15 PYGB S15 GYS1CK1MAPKAPK2DYRK1ADYRK2DYRK1B Glycogen Regulation AMPKa  PKA S11 S641 S645 cAMP S8 GYS2 S641 S8 S82 Glycogen DegradationGlycogen Synthesis AMP Ca 2+ Grk3PASK GSK3 

6. S645 p38  MAPKPHK  1 PYGM S15 PYGL S15 PYGB S15 GYS1CK1MAPKAPK2DYRK1ADYRK2DYRK1B Glycogen Regulation AMPKa  PKA S11 S641 S645 cAMP S8 GYS2 S641 S8 S82 Glycogen DegradationGlycogen Synthesis AMP Ca 2+ Grk3PASK GSK3 

7. Glycogen Regulation www.phosphoNET.ca

8. Glycogen Regulation www.phosphoNET.ca

9. Glycogen Regulation www.phosphoNET.ca Substrate Short Name Substrate Full NameProteinTypeSubstrate Swissprot Phospho- site Effect of Phosphorylation Kinase Affecting All Kinases Affecting Phosphatase Affecting GYS2Glycogen synthase, liverGlygcogen synthesis enzyme P54840S8Phosphorylation inhibits enzyme activity. PKACaAMPKa1, CK1, MAPKAPK2, PhK- , PKACa PHKG1Phosphorylase B kinase gamma catalytic subunit 1 Glygcogen regulation enzyme Q16816S82Phosphorylation may stimulate kinase activity. PKACa PYGBGlycogen phosphorylase, brain form Glygcogen degradation enzyme P11216S15Glycogen phosphorylase, brain form PhK-g PYGLGlycogen phosphorylase, liver form Glygcogen degradation enzyme P06737S15Glycogen phosphorylase, liver form PhK-g PYGMGlycogen phosphorylase, muscle form Glygcogen degradation enzyme P11217S15Glycogen phosphorylase, muscle form PhK-g