1. Denaturation temperatuare gradient- polymerase chain reaction: by-product? Ji Youn Lee Cell and microbial engineering laboratory Seoul National University

2. Let ’ s talk about DTG-PCR A newly invented tool by modifying the typical PCR for solving traveling salesman problem More amplification of DNA strands with lower melting temperature Is it True? Does it operate Well? Is it Worth?

3. It ’ s time to study DTG-PCR Experimental part Modeling and simulation part Make use of the published ones : Competitive PCR Addition of the ssDNA distribution profile with temperature

4. Let ’ s think about it!

5. Experimental considerations DNA strands with different Tms –Same length, different Tm –or Different length, different Tm –or Different length, same Tm … etc. Experiments –Mix the strands (two or more) –Perform DTG-PCR in various conditions –Analysis: TGGE, DGGE or graduate PCR

6. Theoretical considerations Studies up to date –Competitive PCR –Quantitative PCR –Internal standards and products –Anal Biochem (1993) Quantitative PCR: Theoretical considerations with practical implications Replication efficiency E (product and standard)

7. –NAR (1996) A mathematical model and a computerized simulation of PCR using complex templates Probability calculation –PNAS (1996) Efficiency of DNA replication in the PCR –NAR (1997) A coalescent approach to the PCR –NAR (1998) Modeling and analysis of competitive RT–PCR

8. –JTB (1997) Enzymological considerations for a theoretical description of the quantitative competitive PCR Michaelis-Menten equation (enzyme kinetics) –JTB (1997) Theoretical description of the PCR –JTB (1999) Polymerase chain reaction: Markove process approach –Bioinformatics (2001) Virtual PCR