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NYKRCP004 RT- PCR report 2009. 09. 29 1. PCR Condition Temp.TimeCyclesFinal Reaction vol. 45 ℃ 30min1 50ul 95 ℃ 15min 1 95 ℃ 30sec 35 54 ℃ 30sec 72 ℃ 40sec.

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Presentation on theme: "NYKRCP004 RT- PCR report 2009. 09. 29 1. PCR Condition Temp.TimeCyclesFinal Reaction vol. 45 ℃ 30min1 50ul 95 ℃ 15min 1 95 ℃ 30sec 35 54 ℃ 30sec 72 ℃ 40sec."— Presentation transcript:

1 NYKRCP004 RT- PCR report 2009. 09. 29 1. PCR Condition Temp.TimeCyclesFinal Reaction vol. 45 ℃ 30min1 50ul 95 ℃ 15min 1 95 ℃ 30sec 35 54 ℃ 30sec 72 ℃ 40sec 72 ℃ 10 min 1 4℃4℃ Storage 1 2. Primer set NAMEGENESize (bp) Pr7-F ORF7 FULL 662 Pr7-R NA-Pr5-F NA ORF5 FULL 716 NA-Pr5-R EU-Pr5-F EU ORF5 FULL 755 EU-Pr5-R NSP2-F NSP2 PARTIAL N:768 H: 678 NSP2-R

2 NYKRCP004 RT- PCR report 2009. 09. 29 #sample260/280260/230conc.(ng/ul) 1I3-09-001532.041.37291.6 2I3-09-001542.031.38260.4 3I3-09-001552.012.03294.2 4I1-09-001602.052.241111.5 5I1-09-001612.052.261389.7 6I1-09-001622.062.151734.7 7I1-09-00164/1652.441.581.9 8I1-09-00166/1671.330.551.9 9I1-09-00168/1693.490.442.5 3. Sample list

3 3. The Result of PCR 2009. 09.29 NYKRCP004 RT- PCR report 1 kb 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 #GenePrimer setSize(bp) 1 ORF7 FULLPr7 F/R662 2NA ORF5 FULLNA-Pr5 F/R716 3NSP2 PARTIALNSP2 F/R768 500bp 1kb #sample 1I3-09-00153 2I3-09-00154 3I3-09-00155 4I1-09-00160 5I1-09-00161 6I1-09-00162 7I1-09-00164/165 8I1-09-00166/167 9I1-09-00168/169 NA-Pr5 Pr7 NSP2

4 NYKRCP004 RT- PCR report 2009. 09. 29 4. RNA gel loading lanesampleconc.(ng/ul) Loading 양 1I3-09-00153291.65ul 2I3-09-00154260.45ul 3I3-09-00155294.25ul 4I1-09-001601111.55ul 5I1-09-001611389.75ul 6I1-09-001621734.75ul C 1 2 3 4 5 6 C->Control RNA 1.4ug

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