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PrimerSequence (5' to 3')Comments Hpt-FwCGCGACGTCTGTCGAGAAGTTT RT-PCR analysis Hpt-RvATCGGACGATTGCGTCGCATCGA RT-PCR analysis AOX1a-FwGATGTTTGTCTACTGCCGAGGATTT.

Published byAgatha Dixon Modified over 9 years ago

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Presentation on theme: "PrimerSequence (5' to 3')Comments Hpt-FwCGCGACGTCTGTCGAGAAGTTT RT-PCR analysis Hpt-RvATCGGACGATTGCGTCGCATCGA RT-PCR analysis AOX1a-FwGATGTTTGTCTACTGCCGAGGATTT."— Presentation transcript:

1 PrimerSequence (5' to 3')Comments Hpt-FwCGCGACGTCTGTCGAGAAGTTT RT-PCR analysis Hpt-RvATCGGACGATTGCGTCGCATCGA RT-PCR analysis AOX1a-FwGATGTTTGTCTACTGCCGAGGATTT RT-PCR analysis AOX1a-RvATGTAGTATATATAACTCAGCTGCC RT-PCR analysis AOX1b-FwTCATCATTCATCAACGGGCGATGC RT-PCR analysis AOX1b-RvTGTGCACGGGTCAGCCAACGGCCA RT-PCR analysis AOX1c-FwCTGAAGAAATCTTACGGCGG RT-PCR analysis AOX1c-RvCCAAACAGATAACAGGACGC RT-PCR analysis SpeI-AOX1A-NdeI-FwACTAGTATGAGCTCCCGGATGGCCG Plasmid construction for overexpression SpeI-AOX1A-NdeI-RvCATATGGTGATATCCGATCGGCGCAGG Plasmid construction for overexpression Actin1-FwATGCTCTCCCCCATGCTATC RT-PCR analysis Actin1-RvTCTTCCTTGCTCATCCTGTC RT-PCR analysis Supplementary Table 1. Primers used in this study. Table S1

2 Fig. S1 (a) (b) (c) (e) (f) 0 1 2.5 5 10 25 50 SOD activity (units mg -1 protein h -1 ) 0 200 400 600 800 1000 1200 1400 1600 1800 a a a b b b b (d) GR activity (unit mg -1 protein) Time (h) 0 20406080 0.01 0.02 0.03 0.04 0.05 0.06 0.07 Hyg 0 μ M Hyg 25 a b c c c c c μ M APX activity (unit mg -1 protein) Time (h) 0 20406080 0.0 0.5 1.0 1.5 2.0 2.5 3.0 Hyg 0 Hyg 25 a b b b b b b μ M μ M Time (h) 020406080 200 300 400 500 600 700 Hyg 0 Hyg 25 SOD activity (unit mg -1 protein h -1 ) a b cd c μ M μ M 0 1 2.5 5 10 25 50 0.0 0.5 1.0 1.5 2.0 2.5 3.0 APX activity (units mg -1 protein) Hyg conc. ( a a b b b b b μ M ) Hyg conc. ( 0 1 2.5 5 10 25 50 0.00 0.02 0.04 0.06 0.08 0.10 GR activity (unit mg -1 protein) Hyg conc. ( a a b b b b b μ M ) μ

3 (a) AOX1a 03612240361224 Control Hyg (hr) Actin1 AOX1b AOX1c (b) AOX 01248720124872 Control Hyg (hr) 34 kD 32 kD CBB Fig. S2

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