1. Help: Strain Page Header Yeast ORF deletion: _d suffix : dubious ORF _p suffix : putative (uncharacterized) ORF Gene/Protein: The established name for this ORF, if available Description : A description of the protein/gene from SGD Zygosity : Whether the deletion strain has been screened in a heterozygous (HIP) or homozygous (HOP) background. SGD Linkout: Link to the SGD page for this ORF Yeast ORF deletion: _d suffix : dubious ORF _p suffix : putative (uncharacterized) ORF

2. Help: Strain Fitness Profile Fitness Plot: Fixed View Fitness axis covers a fixed range (-5 to 12.5) Data points outside fixed range are captured in upper/lower rescaled areas (in grey) Fitness Plot: Expanded View Fitness axis expands to cover full range of data Fitness Axis Fitness value for this deletion strain under the given screening conditions Growth Condition Axis Screen Condition, arranged numerically by SGTC_ID Significant Fitness Defect Scores Highlighted in Yellow. These are clickable to show details on the screen and a link to the screen profile Information Bubble SGTC Screen Identifier, with link to Screen Profile Screening conditions for this point Fitness Defect Score P-value View Selector Fixed or Expanded View

3. Help: Strain Fitness Table This table provides details on the screens which generated high fitness defect scores. The top 20 are listed, ranked by fitness defect score. Screen Details Rank : By FD Score, and shown on the plot Screen ID : The SGTC_ID assigned to this screen Condition : Compound (and other conditions) FD Score : the FD score for this deletion strain under the given condition Conc : the concentration of compound screened. P-value : from the distribution of fitness scores from all screens for this deletion strain Strain Behaviour within screen Rank : By FD Score, the rank of this strain among all strains in the given screen conditions. Screen P-value : from the distribution of all fitness scores for all strains in the given screen conditions. Screen Clearance : see methods for details. Access to full data set Either as a tab-delimited text file, or excel table.

4. Help: Cofitness Table This table provides details on the deletion strains with similar patterns of inhibition across screens. The top 15 with positive correlations are listed, ranked by p-value. Access to full data set Either as a tab-delimited text file, or excel table. Cofit Details Correlation : The correlation score calculated between fitness defects scores across screens for the query strain and this deletion strain. See methods for details on calculation. pval : see methods for details ORF : the ORF deleted Gene : the associated Gene/Protein. Links to the fitness profile for this deletion strain. Zygosity : heterozygous (HIP) or homozygous (HOP) Description : A description of the gene, from SGD

5. Help: Screen Page Header Screened Compound (and conditions) Often a library ID. Links to pubchem IUPAC name Compound Description When available Relevant Links PubChem : http://pubchem.ncbi.nlm.nih.gov/ DrugBank : http://www.drugbank.ca/ Wikipedia : http://en.wikipedia.org/ WikiGenes : http://www.wikigenes.org/ ChEMBL : https://www.ebi.ac.uk/chembl/ ChemSpider : http://www.chemspider.com/ MeSH terms, from PubChem Chemical Structure Image FDA approved indicator Screen Information: Exp ID : unique SGTC identifier Concentration Source : Library Source for this compound CID : Links to pubchem SMILES string Molecular Weight ALogP : Partition coefficient H-bond Donor/Acceptor Counts Pool Growth Kinetics: Relative growth inhibition, calculated separately for the homozygous and heterozygous deletion pools grown under these screening conditions vs control conditions

6. Help: Screen Fitness Profile Fitness Plot: Fixed View Fitness axis covers a fixed range (-5 to 12.5) Data points outside fixed range are captured in upper/lower rescaled areas (in grey) Fitness Plot: Expanded View Fitness axis expands to cover full range of data Fitness Axis Fitness value for this deletion strain under the given screening conditions Deletion Strain Axis Arranged alphabetically by Gene name Significant Fitness Defect Scores (labelled with gene) Highlighted in Yellow. These are clickable to show details on the screen and a link to the strain profile Information Bubble Gene Symbol + ORF, with link to strain profile Fitness Defect Score P-value Clearance Value Gene Description View Selector Fixed or Expanded View Profile Type HIP : heterozygous strains HOP : homozygous strains HIP Target Highlighted in red. Determined to be a target for this screening compound. See methods. Access to full data set Either as a tab-delimited text file, or excel table. Frequent Flyer Marked with a cross. Deletion strain is frequently seen with a fitness defect across screens.

7. Help: Fitness Table These tables provide details on the strains which highest fitness defect scores in this screen. The top 15 are listed, ranked by fitness defect score, for each of the heterozygous (HIP) and homozygous (HOP) subpool. Strain Details Deletion Strain : Systematic ORF name. Suffixes _d = dubious ORF, _p = putative(uncharacterized) ORF FD Score : the FD score for this deletion strain under the given condition P-value : from the distribution of fitness scores from all deletion strains in this subpool/screen Clearance : for HIP only, see methods. Gene : the gene/protein name for this deleted ORF. Links to the strain profile. Description : from sgd Access to full data set Either as a tab- delimited text file, or excel table. Subpool Identification HET : Heterozygous strains (HIP) HOM : Homozygous strains (HOP)

8. Help: GO Enrichment Analysis Enriched GO terms were determined from the fitness defect scores across all strains under these screening conditions. These are presented as interactive network maps which can be explored through the links provided (or directly in cytoscape). Links, wil be available if enrichment has been determined in these categories Biological Processes : Protein Complexes