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Histology.

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Presentation on theme: "Histology."— Presentation transcript:

1 Histology

2 Light Microscopy (LM) – paraffin embedded, 7 micron sections, specific stains
Transmission Electron Microscopy (TEM) – plastic embedded, 70 millimicron sections, e-dense stains Scanning Electron Microscopy (SEM) – critically point dried, gold coated

3 Light Microscopy

4 TEM

5 SEM

6 Epithelium Simple Epithelium

7 Simple Squamous Mesothelium

8 Simple Squamous (Mesothelium) by TEM

9 Simple Squamous – Endothelium (En)

10 Simple Squamous – Endothelium (Ed) by TEM

11 Capillary (endothelial cell) by TEM

12 Simple Cuboidal Epithelium (longitudinal section)

13 Simple Cuboidal (cross section)

14 Simple Cuboidal by TEM

15 Simple Columnar with Goblet Cells

16 Simple Columnar (cross section)
with Goblet Cells (arrow)

17 Goblet Cell with PAS stain (red)

18 Simple Columnar with Goblet Cells (Mu) by TEM

19 Simple Columnar (AC) with Goblet Cells (GC) by SEM

20 Simple Columnar mucus secreting

21 Simple Columnar mucus secreting

22 Stratified Epithelium

23 Pseudostratified Ciliated Epithelium with Goblet Cells

24 Pseudostratified Epithelium with Cilia (a) and Goblet Cells (b)

25 Stratified Squamous non-Keratinized Epithelium

26 Transitional Epithelium

27 Transitional Epithelium with rounded binucleated surface cells

28 Stratified Squamous Keratinized (thin)

29 Stratified Squamous Keratinized (thick)

30 Epithelial Cell Junctions

31 Tight Junctions (Zonula Occludens) by TEM

32 Tight Junctions (Zonula Occludens) by Freeze Fracture

33 Gap Junction (Nexus) by TEM

34 Gap Junction Connexin molecules 2 nM gap between membranes
Open pores for ions, molecules, etc

35 TEM of Zonula Adherens (red arrows)
TEM of a Desmosome (blue arrow)

36 TEM (colorized) of Adherens Junctions

37 TEM of a Desmosome with tonofibrils

38 TEM of a Hemi-Desmosome

39 Summary of the maintenance of cell shape by junctions and cytoskeletal elements

40 Glandular Epithelium

41 Serous Gland with ducts (arrows)

42 Serous secretory unit (high magnification)

43 Mucous Gland with duct (arrow)

44 Mixed Gland with Serous Demilunes (SD)


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