1. AP Lab Skills Guide

2. AP Lab Skills Guide

3. + AP Lab Skills Guide Mix contents Begin Timing
Tube 1 (substrate) Tube 2 (enzyme)
Tube 3
(tubes 1 + 2)
Begin Timing
H2O2
Guaiacol
Distilled H2O
Peroxidase
Distilled water

4. AP Lab Skills Guide
Each minute for 5 min compare the color of tube 3 to the standard
Time (min)
Color 1 2 3 4…
Tube 3
(tubes 1 + 2)
Begin Timing
Graph the data…

5. AP Lab Skills Guide
Time (min)
Color 1 2 3 4 5 6 7 8 10
Each minute for 5 min compare the color of tube 3 to the standard
You are determining how long to let the reaction go to get a value of “5” as your baseline for comparisons in the next step…you will see…we will run all future reactions for 5 minutes and look at color and compare to enzyme in distilled water….

6. AP Lab Skills Guide

7. AP Lab Skills Guide
Tube 1
Tube 2
Tube 3
Tube 4
Tube 5
Tube 6
Peroxidase
H2O2
pH 12 buffer
Peroxidase
H2O2
pH 2 buffer
Peroxidase
H2O2
pH 4 buffer
Peroxidase
H2O2
pH 6 buffer
Peroxidase
H2O2
pH 8 buffer
Peroxidase
H2O2
pH 10 buffer
Cover with parafilm, gently mix and measure color at 0 minutes and color change at time determined in part 1 (5 minutes)...

8. AP Lab Skills Guide pH
Color 2 1 4 6 5 8 3 10 12
Of course, we should combine the class data, take averages, SD, SE and graph with error bars…

9. AP Lab Skills Guide

10. AP Lab Skills Guide

11. AP Lab Skills Guide

12. AP Lab Skills Guide

13. AP Lab Skills Guide