1. Excellent ed maps can be obtained by MAD phasing.

2. Electrophoretic mobility Frictional coefficient Polyacrylamide gel Use fresh ammonium persulfate.

3. SDS-PAGE Stacking gel Resolving gel Discontinuous buffer system

4. 1. pI (isoelectric focusing) 2 Mr (SDS-PAGE) log Mr SDS- PAGE

5. MWCO of dialysis tubing

6. DEAE-CM- affinity column Concanavalin A Gel filtration column: long Ion exchange column: medium Affinity column: small

7. Sedimentation velocity Centrifugal force Sedimentation coefficient 1 S=10 -13 sec

8. Mass under nondenaturing conditions

9. ESI Mass Spectrum MALDI TOF Matrix Assisted Laser Desorption Ionization Time-of-Flight

11. One cycle of Edman degradation Mixture of PTH-aa ’ s

14. Performic acid oxidation and cleavage of -S-S- N-terminal sequencing Degenerate oligomer primer cDNA cloning DNA sequencing

16. 4 Ca 2+ binding motifs in calmodulin Hybridoma cell

17. ELISA

18. Western blotting Imaging plate (IP) is 50-times more sensitive than X-ray film. Actin filaments

19. Gold cluster-conjugated antibody Secondary structure content

21. Low resolution High resolution

22. Spin =1/2

23. When R < 5 A R Chemical shift

24. X-ray vs NMR

25. DCC 항이뇨성의 Natural Synthetic

26. Merrifield solid-phase peptide synthesis