1. Eric Niederhoffer SIU-SOM What is the role of the pentose phosphate pathway?

2. Overview NADPH production Glutathione reduction (E), fatty acid/cholesterol synthesis (L) Glycolytic intermediates Fructose-6-phosphate, glyceraldehyde-3-phosphate Ribose-5-phosphate Nucleotide synthesis (nucleated cells)

3. Glycolysis and Pentose Phosphate Pathways PPP NADPH 6PG 3-7 C metabolites (R5P, F6P, G3P) G6PDH lactonase 6PGDH CO 2 NADP + + H + GSH GSSG GR GP H2O2H2O2 H2OH2O Glc Pyr G6P 1,3-BPG 3PG HK PGI PK F6P DHAP + G3P PFK aldolase F16BP 2PG PEP PGK PGM enolase G3PDH Glycolysis

4. Oxidative Phase GlucoseG6P HK F6P PGI G6PDH 6PGL NADPH NADP + lactonase 6PG FA/cholesterol synthesis RBC ox stress CO 2 6PGDH Ru5P NADPH NADP +

5. Non-oxidative Phase Ru5P Xu5P RuP3E R5P R5PI TK S7P G3P TA E4P F6P TK F6P G3P Glycolysis Nucleotides

6. Review Questions What are the main functions of the pentose phosphate pathway? Which enzymes produce NADPH? Which intermediates can feed back into the glycolytic pathway?