1. What invention helped with many discoveries in science? Agenda for Thursday Sept 8 th 1.Microscopes 2.Lab Learning Objectives Identify microscope parts and know their function Compute magnification for a microscope Measure the size (in microns) of objects under the microscope

3. Basic Rules Carry using arm and base Flat Surface Adjust light using diaphragm Use stage clips Focus with course adjustment on low and medium power only! – High Power use fine adjustment only

4. Magnification Total magnification = eyepiece magnification X objective magnification Eyepiece is 10x and objective is 10x what is total magnificaion?

5. How to make a wet mount slide 1.Specimen on slide 2.Drop of water/stain 3.Cover slip on slide

6. Calculate Field of View Clear Ruler Determine how many millimeters across Convert to micrometers 1mm=1000μm So: if 1.5 mm across then field of view is 1.5mm x 1000 μm/1mm = 1500 μm

7. Calculate Size of Specimen Figure out how many will fit across the field of view (estimate) Divide field of view length by how many fit across – If 8 fit across and field of view is 800 μm then specimen is 100 μm – 800 μm/8 = 100 μm

8. Remember! Carry using arm and base Flat Surface Adjust light using diaphragm Use stage clips ONLY USE FINE ADJUSTMENT ON HIGH POWER!

9. Objectives Identify microscope parts and know their function Compute magnification for a microscope Measure the size (in microns) of objects under the microscope

10. What is the function of the diaphragm on the microscope? Agenda for Friday Sept 9 th 1.Microscope lab Learning Objectives Identify microscope parts and know their function Compute magnification for a microscope Measure the size (in microns) of objects under the microscope

11. What is the total magnification if you have a 10x eyepiece and are using a 40x objective? Agenda for Monday Sept 12 th 1.Microscope Quiz 2.Microscope Lab Learning Objectives Identify microscope parts and know their function Compute magnification for a microscope Measure the size (in microns) of objects under the microscope

12. Low power = 4000 μm Medium power = 1500 High power = 500