1. Do Now—5.6.15
What is personal protective equipment (PPE)?
What do you think it means for something to be sterile? Clean?

2. Unit 5: Infectious Diseases 5.1.6:Lines of Defense

3. Objective
SWBAT reflect on their performance on the group lymphatic system assessment.
SWBAT diagram the steps necessary to isolate bacteria.

4. Nonspecific vs. Specific WS
Nonspecific external defense mechanisms—these keep pathogens from entering the body without pathogen recognition.
Both: Nonspecific external defense (first defense)—keeps pathogens out
Skin: block pathogen entry, suppresses microbe growth. Mucus: traps pathogens

5. Nonspecific vs. Specific WS
Nonspecific internal defense mechanisms (phagocytic cells, inflammation)—these kill pathogens after they have been able to enter the body.
WBCs indicate that there is a serious infection because WBCs are phagocytic cells, which are part of nonspecific and specific immunity. WBCs are involved in inflammation and cell-mediated immunity. WBCs engulf pathogens.

6. Nonspecific vs. Specific WS
Don’t have notes for #6 and #7, but these are the answers

7. Nonspecific vs. Specific WS
Bacteria, virus, allergens (dust, pollen, etc —you’ll see in the Immunity packet)
Cell-mediated response involves the T cells (helper and cytotoxic) and macrophages.
Antibody-mediated response (humoral response) involves the B cells (plasma and memory) and antibodies and can involve helper T cells.

8. Nonspecific vs. Specific WS
The clump (antigen and antibody interaction) attracts phagocytes (WBCs) and the WBCs engulf the clump and destroy it.

9. Nonspecific vs. Specific WS
The clump (antigen and antibody interaction) attracts phagocytes (WBCs) and the WBCs engulf the clump and destroy it.
The clump (antigen and antibody interaction) attracts phagocytes (WBCs) and the WBCs engulf the clump and destroy it.

10. Nonspecific vs. Specific WS
The clump (antigen and antibody interaction) attracts phagocytes (WBCs) and the WBCs engulf the clump and destroy it.

11. Nonspecific vs. Specific WS
Inflammatory Response
4 –
5 –
8 –
3 –
2 –
7 –
1 –
6 -
WBCs
Cytotoxic T cells
B cells
Helper T cells
Plasma B cells

12. 5.1.3: Isolating Bacteria

13. 5.1.3: Isolating Bacteria
Aseptic technique is a method of preventing the transmission of infectious agents to/from specimens; working in sterile (no infectious agents) conditions.

14. 5.1.3: Isolating Bacteria
New sterile plastic inoculating loops must be used to transfer bacterial cultures. Metal inoculating loops can be sterilized (using fire) in between cultures.

15. 5.1.3: Isolating Bacteria
New sterile plastic inoculating loops must be used to transfer bacterial cultures. Metal inoculating loops can be sterilized (using fire) in between cultures.
Caps of test tubes or tops of Petri dishes must be held during the entire procedure and never placed on the table or contamination will result.

16. 5.1.3: Isolating Bacteria
New sterile plastic inoculating loops must be used to transfer bacterial cultures. Metal inoculating loops can be sterilized (using fire) in between cultures.
Caps of test tubes or tops of Petri dishes must be held during the entire procedure and never placed on the table or contamination will result.
Proper personal protective equipment needs to be worn at all times.

17. 5.1.3: Isolating Bacteria
Absolutely no food or drink is allowed in the laboratory area.

18. 5.1.3: Isolating Bacteria
Absolutely no food or drink is allowed in the laboratory area.
All work surfaces need to be disinfected before and after the lab.

19. 5.1.3: Isolating Bacteria
Absolutely no food or drink is allowed in the laboratory area.
All work surfaces need to be disinfected before and after the lab.
Hands must be thoroughly washed with soap and water before and after working with any samples.

20. 5.1.3: Isolating Bacteria
Streak plating is a method used to obtain one individual bacteria colony.

21. 5.1.3: Isolating Bacteria
Streak plating is a method used to obtain one individual bacteria colony.

22. Homework
STEM Survey---late credit 75/100 if you complete it for Thursday. 50/100 if by Friday.
Quiz on Unit 5 on Tuesday – start studying and coming to tutoring for help!

23. 5.1.3: Isolating Bacteria

24. 5.1.3: Isolating Bacteria

25. 5.1.3: Isolating Bacteria

26. 5.1.3: Isolating Bacteria

27. 5.1.3: Isolating Bacteria

28. 5.1.3: Isolating Bacteria

29. 5.1.3: Isolating Bacteria

30. Homework
STEM Survey---late credit 75/100 if you complete it for Thursday. 50/100 if by Friday.
Quiz on Unit 5 on Tuesday – start studying and coming to tutoring for help!