1. The NucleoCounter® NC-100™ Fast, precise and objective counting and viability determination of mammalian cells

2. NucleoCounter® NC-100™ key advantages and selling points Easy operation – no preparation 30 sec. analysis time Calibration free No cleaning Maintenance and service free Portable/compact Safe sample handling and disposal High precision & reproducibility

3. NucleoCounter® NC-100™ - Mammalian cells NucleoCounter is optimized to count mammalian cells cultured in TC-flasks, bioreactors, micro carriers etc.

4. NucleoCounter® NC-100™ Concept: 5 components NucleoCounter® NC-100 ™ NucleoCassette Lysis Buffer Stabilizing Buffer NucleoView Software

5. Easy Operation LyseLoad Count

6. Propidium Iodide 1 dye molecule per 4-5 bp Genome between 2.5 x 10 9 – 4.0 x 10 9 bp. DNA base pair (bp): A: Adenine T: Thymine G: Guanine C: Cytosine C 27 H 34 N 4 2+.2I - Mr 668.4

7. Limitations of present methods of cell counting

8. Cell Counting, Whole Nuclei Using Fluorescent DNA Dye PI Hard to discriminate cells from debris by microscopy Easy to count nuclei by fluorescence microscopy

9. Integrated Fluorescence Microscope Lenses: magnification <1X. Excitation filter: sorts out green light. Emission filter: sorts out long wave length including red light. fixture

10. Integrated Fluorescence Microscope

11. The NucleoCassette™

12. Nuclei of Mammalian cells Different in cell size: Diameter between 20-40  m. Nuclei are almost uniform in size: 10-12  m. Genome between 2.5x10 9 – 4.0x10 9 bp. Advantage: No calibration to cell type (size) using the NucleoCounter NC-100.

13. Reagent A-100 and B Lysis reagent A-100: Disrupts the plasma membranes of the mammalian cells, rendering the nuclei susceptible to staining with PI Disaggregation of cell clusters Stabilizing reagent B: Raises the pH value, allowing PI to stain the DNA of the cells more efficiently

14. Viability Determination

15. NucleoCounter® NC-100™ comparison with conventional cell counting methods

17. NucleoView  Software

18. Technical Specifications

19. Validation IQ/OQ

20. Typical NC-100 customers

21. NucleoCounter® NC-100™ key advantages and selling points Easy operation – no preparation 30 sec. analysis time Calibration free No cleaning Maintenance and service free Portable/compact Safe sample handling and disposal High precision & reproducibility