1. Femtosecond dynamics and near-field microscopy of chemical and biological objects A.N. Petrukhin, O.M. Sarkisov Institute of Chemical Physics Moscow, Russia

2. 1.Femtosecond dynamics of bifunctional molecules. 2.Near-field microscopy of lipofuscin granules from human eyes.

3. Photochromic reactionESIPT reaction C-O bond cleavage: 250 - 400 fs Formation of intermediate X: 300-450 fs Formation of colored photoproduct (550-600 nm): 1 - 3 ps Excited state intramolecular proton transfer (ESIPT): < 80 fs Formation of twisted structure: 0.3 – 4.4 ps Formation of colored photoproduct (480-500 nm): 3.8 – 14 ps S.A. Antipin, A.N. Petrukhin, F.E. Gostev, V.S. Marevtsev,A.A. Titov, V.A. Barachevsky, Yu.P. Strokach, O.M. Sarkisov, Chem. Phys. Lett. 331 (2000) 378. F.E. Gostev, A.N. Petrukhin, A.A. Titov, V.A. Nadtochenko, A.V. Metelitsa, V.I. Minkin, O.M. Sarkisov, Rus. Chem. Bul. 2004. 23, 11 3-14. h

4. Bifunctional molecules: compounds 1-3 1: 2: 3: Does proton transfer take place?

5. Pump: 405 nm, 40 fs Probe: Supercontinuum, 410-800 nm Experimental setup

6. Photoinduced absorption dynamics

8. Product yield ratio dependence on pump energy Compounds 1,2 S0S0 S 1 ** S 1 K* S1KS1K S0KS0K S1*S1* XB B S0KS0K S0KS0K B 120 fs 800 fs 20 ps 400 fs 3 ps B – photochromic product S 0 K – ESIPT product ESIPT C-O cleavage

9. Product yield ratio dependence on pump energy Compound 3 S0S0 S 1 ** S 1 K* S1KS1K S0KS0K S2*S2* XB 850 fs3 ps 450 fs 21 ps B S0KS0K S0KS0K B 120 fs B – photochromic product S 0 K – ESIPT product C-O cleavage ESIPT

10. Compound 1. Kinetic curve at 510 nm Pump pulse chirp influence on wave packet motion

11. Pump pulse chirp influence on product yield ratio Time delay = 200 ps

12. 2. Near-field microscopy of lipofuscin granules from human eyes.

13. Near-field microscopy. Experimental Setup.

14. 200 nm Single LF granule. Atomic-force microscopy. Topography Phase contrast

15. Single LF granule. Near-filed microscopy. Topography Transmission at 410 nm

16. Single LF granule. Near-filed microscopy. Topography Fluorescence spectra

17. Two photon microscopy of LP granules

18. Fluorescence decay of LH granules under 400 nm fs irradiation Fluorescence decay – epoxide formation?

19. Prof. Oleg Sarkisov Prof. Victor Nadtochenko Andrey Titov Fyodor Gostev Andrey Petrukhin Andrey Frolov Alexander Sobennikov Arseniy Aybushev Artem Astafyev Ivan Shelaev Pavel Zolotavin Institute of Chemical Physics Moscow, Russia Thank you very much! Laser spectroscopy Lab femto.chph.ras.ru +7-095-939-7162 Our team: