1. Pathogenic spirochetes. Chair of Medical Biology, Microbiology, Virology, and Immunology

2. Spirochetes Treponema Borrelia Leptospira

3. Main pathogenic bacteria of Spirochetaceae GenusSpeciesSubspeciesDisease Treponema T.pallidum T.сarateum T.vincentii pallidum endemicum Pertenue Syphilis Bejel Yaws Pinta Vincent’s angina Borellia B.recurrentis B.caucasica, B.duttoni, B.persica та ін Epidemic relapcing fever Endemic relapsing fever B.burgdorferiLyme disease Leptospira Icterohaemo- rrhagiae Leptospirosis

4. Spirochetes structure

5. Spirochetes morphology Treponema Leptospira Borrelia

6. Spirochetes ultrastructure Borrelia Leptospira

7. Classification of Human Terponema  Non-pathogenic T.denticola T.macrodenticum T.orale  Conditionally pathogenic T.vincentii  Pathogenic T.pallidum pallidum (syphilis) T.pallidum endemicum (bejel) T.pallidum pertenue (yaws) T.carateum (pinta)

8. Vincent’s angina

9. Noma (gangrenous stomatitis)

10. Treponema in tested material

11. Cell attacked by treponema

12. Hard chancre

13. Clinical findings of Syphilis Secondary syphilis Hetchinzone teeth

14. Microbiologic diagnosis of Syphilis  Microscopy (native an fixed material) Romanovsky-Giemsa stain Phase contrast Dark field  Serologic diagnosis Wassermann’s test (CFT) Sedimentation reartions (Каhn’s and Sachs- Witebsky’s tests) Microreaction of Treponema pallidum immobilization IFT, ELYSA, IHAT Chain polymerase reaction

15. Tropic treponematoses: pinta (T.carateum), bejel (T.endemicum), yaws (T.pertenue) Yaws (tropic granuloma) Bejel (endemic syphilis)

16. Yaws

17. Pinta

18. Bejel

19. Borrelia morphology

20. Borrelia in blood smear

21. Lyme disease Causative agents: Borrelia burgdorferi Borrelia garinii Borrelia afzelii

22. B. burgdorferi

23. Leptospira morphology

24. Clinical findings of leptospirosis