1. Quantitation part 9
By : Saib Al Owini

2. CELL COUNTING
- Hemocytometer
-Electronic Counting

4. PROTOCOL 21.1. CELL COUNTING BYHEMOCYTOMETER
Outline
Trypsinize a monolayer culture, or sample a suspension culture, prepare a hemocytometer slide, and add the cells to the counting chamber. Count the cells on a microscope and calculate the cell concentration.
Materials
Sterile:
D-PBSA
Crude trypsin, 0.25%
Growth medium
Yellow pipettor tips
Nonsterile:
Pipettor, 20 μL or adjustable 100 μL
Hemocytometer (Improved Neubauer)
Tally counter
Microscope

7. H:\ملفات فيديوأنسجة\YouTube - hemacytometer count- sabrina lin.flv

8. Electronic Counting

9. CASY Electronic Cell Counter.

10. CASY Electronic Cell Counter.

11. Beckman Coulter Vi-CELL Electronic Cell Counter

12. CELL PROLIFERATION
Experimental Design
Growth Cycle

15. Cytotoxicity
Viability

17. H:\ملفات فيديوأنسجة\YouTube - Trypan Blue part 3.flv

19. Metabolic Cytotoxicity Assays
Microtitration Assays