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{ LISST calibration and measurements Aya, Grace, Tom Ocean Optics Summer 2013 Final Project August 2 nd, 2013.

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Presentation on theme: "{ LISST calibration and measurements Aya, Grace, Tom Ocean Optics Summer 2013 Final Project August 2 nd, 2013."— Presentation transcript:

1 { LISST calibration and measurements Aya, Grace, Tom Ocean Optics Summer 2013 Final Project August 2 nd, 2013

2  Calibration  Measurements  Lab  Cruise  Coccolithophore experiment  Instructional Video Outline

3 Calibration: Methodology Calibration relies on agreement between measured and modeled VSF of spherical particles

4 Calibration: Methodology Methodology from Slade and Boss 2006, “Calibrated near-forward volume scattering function obtained from the LISST particle sizer”  Measure spherical beads of known size (for our experiment, sizes were 1μm, 20μm, 50μm) (for our experiment, sizes were 1μm, 20μm, 50μm)  Only use runs in which the relative error of beam c is small  Select data from particular detector rings for different size classes  Exclude the first six rings for 1μm runs  Exclude rings 15 to 32 for 20μm runs  Exclude rings 11 to 32 for 50μm runs

5 Calibration: Coefficients From Slade and Boss 2006Our bead calibration

6 Calibration: Coefficients Our bead calibration Calibration experiments were done with expired beads after using vortex machine on the bottle Expiration dates: April 2007 for 1.0 micrometer March 2012 for 20 micrometer July 2007 for 50 micrometer It still works!

7 Recall… Calibration: Coefficients } Output from LISST data Obtained by bead calibration

8 Measurements: from Lab VSFs for lab measurements

9 PSD for lab measurements: Volume concentration

10 Measurements: from Lab PSD for lab measurements: Number frequency assuming spherical shape

11 Measurements: Cruise

12 VSFs for cruise stations depth = 11m beta (m -1 sr -1 ) angle (radians) depth = 42mdepth = 32m depth = 22m

13 Measurements: Cruise PSDs for cruise stations: volume concentration

14 PSD for Damariscotta River Station: Number frequency assuming spherical shape

15 PSD for Ocean Station : Number frequency assuming spherical shape

16 Investigation of changes in river coccolithophore culture scattering and size distribution pre- and post- acidification  Pre-acidification: pH 9.0  Post-acidification:pH 4.5 Measurements: Coccolithophores http://ina.tmsoc.org/CODENET/GuideImages /COpleurocs/source/40-15.htm

17 Measurements: Coccolithophores Changes in VSF log-loglinear

18 Measurements: Coccolithophores Changes in PSD : volume concentration Mean: 9μm Mean: 8μm

19 Measurements: Coccolithophores Changes in PSD : number frequency assuming spherical shape Median: 5.12 μm Median: 6.14μm

20 Video

21  For just the coccolithophore experiment:  Barney Balch, Meredith White, Dave Drapeau at Bigelow for the coccolithophore culture  Emmanuel Boss for the LISST instrument & instruction  Mary Jane Perry for instruction & supplies  Nathan Briggs for guidance  Larry Mayer for pH paper  Ken Voss for transporting the sample Acknowledgements

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