1. CENTRIFUGE GUIDANCE

2. Background  Most common centrifuge incidents are related to the centrifuge rotors.  All centrifuges are at risk of rotor-related damage.  Contributing factors to rotor-related incidents can include:  Improper use.  Inadequate centrifuge and rotor maintenance.  Age-related metal fatigue.  Centrifuge design issues.

3. Guidance for Lab Managers & EHS Reps Example sign for Sorvall centrifuges  Include centrifuge awareness in lab-specific training.  Create a lab job for centrifuge maintenance.  Ensure centrifuge and rotor manuals are easily accessible to users.  Place appropriate reminder signs on centrifuges.

4.  Create log book to record usage and errors.  Individual runs: Date, speed and length of run, rotor used, user name and lab  Rotor details: Serial number, model number, and age.  Repair and maintenance records  Have centrifuges and rotors routinely inspected for signs of damage and excessive wear. Guidance for Lab Managers & EHS Reps

5. Guidance for Users  Prior to use:  Receive centrifuge-specific training from an experienced user.  Learn how to properly secure rotors and balance tubes.  Refer to centrifuge and rotor manual for information on proper usage.

6.  Using the centrifuge:  Wear appropriate PPE for the hazards used in the centrifuge.  Check the rotor and centrifuge for signs of damage prior to use.  Check compatibility: Contents with tubes, tubes with rotor, and rotor with centrifuge.  Tubes:  Don’t overfill.  Make sure they are balanced. Guidance for Users

7.  Using the centrifuge continued:  Make sure rotor is correctly seated in centrifuge.  Standby until the centrifuge gets to full speed.  Stop the centrifuge if there are any strange noises or shaking.  Record spin details in log book. Guidance for Users

8.  After the spin:  Check for error messages.  Clean up spills inside the rotor and drum to prevent imbalance.  If a problem is suspected:  Put a note on the centrifuge alerting others not to use it.  Report the problem to the lab manager or other appropriate person. Guidance for Users