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CHMI 2227 - E.R. Gauthier, Ph.D. 1 CHMI 2227E Biochemistry I Enzymes: - catalysis
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CHMI 2227 - E.R. Gauthier, Ph.D.2 Enzyme catalysis
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CHMI 2227 - E.R. Gauthier, Ph.D.3 Enzyme catalysis Basic reaction mechanisms General acid/base catalysis: Three main types of catalysis are found: Covalent catalysis: Metal ion catalysis:
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CHMI 2227 - E.R. Gauthier, Ph.D.4 Enzyme catalysis General acid/base catalysis Chymotrypsin O OH R HOR + H
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CHMI 2227 - E.R. Gauthier, Ph.D.5 Enzyme catalysis Metal ion catalysis H+H+
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CHMI 2227 - E.R. Gauthier, Ph.D.6 Enzyme catalysis Covalent catalysis Nucleophile
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CHMI 2227 - E.R. Gauthier, Ph.D.7 Enzyme catalysis Refresher – nucleophiles and electrophiles
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CHMI 2227 - E.R. Gauthier, Ph.D.8 Enzyme catalysis - Enzymes often combine two catalytic strategies General Acid/ base catalysis Covalent catalysis
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CHMI 2227 - E.R. Gauthier, Ph.D.9 Example of enzyme catalysis 1. Chymotrypsin
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CHMI 2227 - E.R. Gauthier, Ph.D.10 Example of enzyme catalysis 1. Chymotrypsin
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CHMI 2227 - E.R. Gauthier, Ph.D.11 Example of enzyme catalysis Importance of the catalytic triad
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CHMI 2227 - E.R. Gauthier, Ph.D.12 Example of enzyme catalysis 1. Chymotrypsin - specificity
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CHMI 2227 - E.R. Gauthier, Ph.D.13 Example of enzyme catalysis 1. Chymotrypsin - specificity Ser 195
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CHMI 2227 - E.R. Gauthier, Ph.D.14 Example of enzyme catalysis 1. Chymotrypsin - catalysis Catalytic triad Substrate C-ter part of the protein N-ter part of the protein Peptide bond cleavage Enzyme regeneration Covalent intermediate
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CHMI 2227 - E.R. Gauthier, Ph.D.15 Example of enzyme catalysis 1. Chymotrypsin - catalysis
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CHMI 2227 - E.R. Gauthier, Ph.D.16 Example of enzyme catalysis Similarities between different proteases
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CHMI 2227 - E.R. Gauthier, Ph.D.17 Example of enzyme catalysis Similarities between different proteases
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CHMI 2227 - E.R. Gauthier, Ph.D.18 Example of enzyme catalysis 2. Enolase
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CHMI 2227 - E.R. Gauthier, Ph.D.19 Example of enzyme catalysis 2. Enolase
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CHMI 2227 - E.R. Gauthier, Ph.D.20 Example of enzyme catalysis 2. Enolase
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CHMI 2227 - E.R. Gauthier, Ph.D.21 Example of enzyme catalysis 3. Lysozyme
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CHMI 2227 - E.R. Gauthier, Ph.D.22 Example of enzyme catalysis 3. Lysozyme Sugar portion of peptidoglycan
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CHMI 2227 - E.R. Gauthier, Ph.D.23 Example of enzyme catalysis 3. Lysozyme
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CHMI 2227 - E.R. Gauthier, Ph.D.24 Example of enzyme catalysis 3. Lysozyme H2OH2O 2
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