1. Group: C1 Ko – Locaylocay
In vitro prebiotic effect of Zingiber officinale (ginger) on Lactobacillus
Group: C1
Ko – Locaylocay

2. Introduction Ginger Zingiber officinale
underground rhizome - herbal medicinal part
Main components:
sesquiterpenoids, nonvolatile phenylpropanoids (gingerol & zingerone), diarylheptanoids
Gingerol – antioxidant, antipyretic, antimicrobial,
antiemetic

3. Introduction Prebiotic
Non-digestible food ingredient – stimulate the growth or activity of one or more bacteria in the colon
Dietary fibers – polysaccharides, lignins & oligosaccharides

4. Introduction Lactobacillus
Gram-positive, facultative anaerobe or strictly aerobic bacteria
Lactic acid bacteria group
Normal flora of vagina, GIT & GUT

5. Significance of the Study
Competitive exclusion principle
Protection against gastric disturbances
Preventive measure for colon cancer

6. Research Question
Does ginger have prebiotic effect on Lactobacillus in vitro?

7. Objectives General Objective:
To determine the prebiotic effect of ginger on Lactobacillus in vitro.

8. Objectives Specific Objectives:
1. To determine whether ginger flesh extract can up-regulate growth of Lactobacillus in in vitro conditions.
2. To determine which ginger extract concentration is the most effective in enhancing the growth of Lactobacillus.
3. To determine the degree and significance of growth up-regulation ginger flesh extracts exert on Lactobacillus in vitro.

9. Methodology

10. Aqueous Ginger Flesh Extract Preparation

11. Aqueous Ginger Flesh Extract Preparation

12. Standardization of Broth Colony and Turbidity Testing

13. Total Bacterial Count

14. Statistical Analysis
ANOVA

15. Group: C1 Ko – Locaylocay
In vitro prebiotic effects of Zingiber officinale (ginger) on Lactobacillus
Group: C1
Ko – Locaylocay