2. You are performing mitosis. Where is this occurring? Describe what is happening.

3. One billion cells/gram The human body consists of a huge number of cells, on the average about one billion cells per gram of tissue. Each cell nucleus contains our entire hereditary material (DNA), located in 46 chromosomes (23 pairs of chromosomes).

4. Mitosis Nuclear division plus cytokinesis – Produces two identical daughter cells – Part of cell cycle

5. Cell Cycle Set of events in cell growth and division Some cells cease to divide and are not considered to be part of the cell cycle. – Examples are: Cells from the heart, eye, and N.S. Cell cycle duration – 8 minutes in some embryos – as long as one year in liver – Most mammalian cells about 24 hours.

6. Cell Cycle Taken from http://ww w.biologyc orner.com /resources /cell_cycle. jpg

7. Stages of Cell Cycle G 1 – Gap 1 S – Synthesis – when DNA is replicated G 2 – Gap 2 M – Mitosis – when chromosomes separate and cytokinesis occurs

8. G1G1 Cells grow in size – Produces RNA and synthesize protein Cells may become quiescence - no longer proliferating – Cells maintain themselves An important cell cycle control mechanism activates (G1 Checkpoint) – It ensures that everything is ready for DNA synthesis.

9. S phase – DNA synthesis Cell replicates genetic material A family of enzymes make an exact replica of the DNA. In human cells, just 6-hours are needed to replicate 46 chromosomes – totalling six billion nucleotides, kept in exact sequence as parental DNA – A mistake = mutation

10. G 2 Phase Cell continues to grow and produce new proteins. At the end of this gap is a control checkpoint (G2 Checkpoint) to determine if the cell can enter M (mitosis) and divide.

11. M Phase - Mitosis Cell growth and protein production All of the cell's energy is focused on the complex and orderly division into two similar daughter cells. Mitosis lasts one to two hours. There is a Checkpoint in the middle of mitosis (Metaphase Checkpoint) that ensures the cell is ready to complete cell division.

12. Regulation/Control of the cell cycle How cell division (and thus tissue growth) is controlled is very complex. – Errors can lead to uncontrolled cell growth – which is how tumors are formed The passage of a cell through the cell cycle is controlled by proteins in the cytoplasm.

13. Control of cell cycle http://www.campbell.edu/faculty/garrett/PHAR%20408/cell%20cycle%20checkpoints.jpg

14. Primary G1/S cell cycle checkpoint

15. Cyclins – Levels rise and fall with the stages of cell cycle. – G1 cyclin (cyclin D) – S-phase cyclins (cyclins E and A) – mitotic cyclins (cyclins B and A)

16. Cyclin dependent kinase Cdks – G 1 Cdk (Cdk4) – S-phase Cdk (Cdk2) – M-phase Cdk (Cdk1) Their levels in the cell remain fairly stable, but each must bind the appropriate cyclin (whose levels fluctuate) in order to be activated. They add phosphate to protein They are the major control switches for the cell cycle, causing the cell to move from G1 to S or G2 to M.

17. Maturation Promoting Factor MPF Triggers progression through the cell cycle p53 – Protein blocks the cell cycle if the DNA is damaged – If the damage is severe this protein can cause apoptosis (cell death). p27 – a protein that binds to cyclin and cdk blocking entry into S phase.