1. Akabane Congenital Arthrogryposis-Hydranencephaly Syndrome, Acorn Calves, Silly Calves, Curly Lamb Disease, Curly Calf Disease, Dummy Calf Disease

2. Overview Organism Economic Impact Epidemiology Transmission Clinical Signs Diagnosis and Treatment Prevention and Control Actions to Take Center for Food Security and Public Health, Iowa State University, 2011

3. THE ORGANISM Center for Food Security and Public Health, Iowa State University, 2011

4. Akabane Virus Single stranded RNA virus –Family Bunyaviridae Genus Orthobunyavirus Sheep, goats and cattle –Intrauterine infection of fetus –No clinical signs in the dam Center for Food Security and Public Health, Iowa State University, 2011

5. IMPORTANCE Center for Food Security and Public Health, Iowa State University, 2011

6. History 1959 –First isolated from mosquitoes in Japan 1969-70 –Israel outbreak –3,000 calves, 700 lambs, 600 kids 1972 –First reported in Australia Center for Food Security and Public Health, Iowa State University, 2011

7. Economic Impact High mortality to newborn and fetal cattle, sheep and goats U.S. livestock greatly susceptible Potential vectors found in U.S. 2010 U.S. livestock statistics –Calves: 35.4 million head –Lambs: 3.6 million head Center for Food Security and Public Health, Iowa State University, 2011

8. EPIDEMIOLOGY

9. Geographic Distribution Tropics and subtropics Australia, Japan, Israel, Korea Occasionally in Asia, the Middle East, and South Africa Center for Food Security and Public Health, Iowa State University, 2011

10. Morbidity/ Mortality Endemic areas –Immunity by sexual maturity –Seroprevalence 80% Greatest risk –Naïve and susceptible animals –Favorable environmental conditions High mortality in newborns –Most die soon after birth or must be euthanized Center for Food Security and Public Health, Iowa State University, 2011

11. TRANSMISSION

12. Animal Transmission Vector-borne –Mosquitoes –Biting midges NOT transmitted by: –Direct contact –Infected tissues, exudates, body fluids –Fomites Ruminants are not long-term carriers Center for Food Security and Public Health, Iowa State University, 2011

13. ANIMALS AND AKABANE

14. Clinical Signs Cattle, sheep, goats Adults asymptomatic Pregnant ruminants –Abortion and stillbirths –Premature births –Dystocia Congenital abnormalities –Varies with stage of gestation Center for Food Security and Public Health, Iowa State University, 2011

15. Congenital Abnormalities Early pregnancy –Behavioral abnormalities “Dummy Calves” –Skull deformities Second trimester –Arthrogryposis (rigidly flexed joints) –Severe muscle atrophy –Torticollis, scoliosis, kyphosis Last trimester –Bright and alert but unable to stand –Ataxia, paralyzed limbs, muscle atrophy Center for Food Security and Public Health, Iowa State University, 2011

16. Post Mortem Lesions Fetuses or newborns Center for Food Security and Public Health, Iowa State University, 2011 ArthrogryposisMicroencephaly and Hydranencephaly

17. Post Mortem Lesions Other congenital abnormalities –Hydrocephalus, microencephaly, spinal cord agenesis or hypoplasia, torticollis, scoliosis, brachygnathism –Cataracts, ophthalmia –Hypoplastic skeletal muscles and lungs –Fibrinous polyarticular synovitis Center for Food Security and Public Health, Iowa State University, 2011

18. Differential Diagnosis Cache Valley virus infection Bluetongue Bovine viral diarrhea virus Border disease Wesselsbron disease Nutritional, genetic, or toxic diseases Center for Food Security and Public Health, Iowa State University, 2011

19. Sampling Before collecting or sending any samples, the proper authorities should be contacted Samples should only be sent under secure conditions and to authorized laboratories to prevent the spread of the disease Center for Food Security and Public Health, Iowa State University, 2011

20. Diagnosis Clinical signs Laboratory Tests –Serology From fetus or neonate Cerebrospinal fluid Adults: antibody titer or seroconversion –Virus isolation and identification –RT-PCR –Immunofluorescent staining Center for Food Security and Public Health, Iowa State University, 2011

21. Treatment No effective treatment Affected neonates should be euthanized Subsequent pregnancies unaffected Center for Food Security and Public Health, Iowa State University, 2011

22. AKABANE IN HUMANS No human cases have been reported

23. PREVENTION AND CONTROL

24. Recommended Actions IMMEDIATELY notify authorities Federal –Area Veterinarian in Charge (AVIC) http://www.aphis.usda.gov/animal_health/area_offices/ State –State veterinarian http://www.usaha.org/StateAnimalHealthOfficials.pdf Quarantine Center for Food Security and Public Health, Iowa State University, 2011

25. Prevention Protection from vectors –Mosquitoes and biting midges Vaccination –Inactivated and attenuated vaccine –Killed vaccine –Not currently available in U.S. Center for Food Security and Public Health, Iowa State University, 2011

26. Additional Resources World Organization for Animal Health (OIE) –www.oie.int U.S. Department of Agriculture (USDA) –www.aphis.usda.gov Center for Food Security and Public Health –www.cfsph.iastate.edu USAHA Foreign Animal Diseases (“The Gray Book”) –www.aphis.usda.gov/emergency_response/do wnloads/nahems/fad.pdf Center for Food Security and Public Health, Iowa State University, 2011

27. Acknowledgments Development of this presentation was made possible through grants provided to the Center for Food Security and Public Health at Iowa State University, College of Veterinary Medicine from the Centers for Disease Control and Prevention, the U.S. Department of Agriculture, the Iowa Homeland Security and Emergency Management Division, and the Multi-State Partnership for Security in Agriculture. Authors: Glenda Dvorak, DVM, MPH, DACVPM ;Anna Rovid Spickler, DVM, PhD Reviewers: James A. Roth, DVM, PhD; Katie Spaulding, BS; Kerry Leedom Larson, DVM, MPH, PhD Center for Food Security and Public Health, Iowa State University, 2011