1. Genome-Wide Identification of Cell Type Specific Transcription Factor Targets During Drosophilia Embyrogenesis Jessica Osterhout

2. How are the same transcription factors used multiple times throughout development to specify dramatically different cell fates? Do they bind and regulate the same target genes in all cases or do they regulates distinct targets in each cell type? Transcription factors to be tested: Eve Hb

3. Chromatin Immunoprecipitaiton followed by sequencing (ChIP-Seq)

5. FLAG Hb Composite HA Hb Composite Hb Antibody Stains Blastoderm: stage 5

6. FLAGHbComposite Stage 16

7. Hb Western Blots Yw Hb Stained with Tub Stained with FLAG

8. FLAGHA Eve Antibody Stains

9. Eve Western Blot

10. To make sure tag does not disrupt Hb protein function: Recombined with two known null alleles to see if tagged Hb rescues.

11. Future Direction: ChIP-Seq with HA or FLAG at each stage of development

12. Acknowledgements and Thanks: SPUR program Mike Miller Chris Doe Khoa Tran Bowerman Lab Prohooda Lab