1. Exercise 2: Review of Microscope Use and Care

2. Review all parts of your brightfield, binocular, compound microscope
Name the three lens systems:
Properly align path of light with Kohler illumination
When viewing specimens, how do you adjust the light properly ?

3. Basic Bacterial Shapes

4. Using the Oil Immersion Lens

5. Two important rules:
Only use oil on 100x objective lens. Never get it onto any of the other objectives!
Never use the coarse adjustment with the 40x and 100x objectives.

6. Trouble Shooting Is the light adjusted properly?
Trouble focusing on the object? Check the following:
Is the light adjusted properly?
Was the object in focus and centered under low power?
Is the oil touching the lens?
Is the lens dirty?
Remember that these objectives are parfocal. If it is out of focus in low power, it will be very out of focus with the others. If you are having trouble focusing the object under low power, focus on a mark placed near the smear.
You will not get a high-resolution image with the oil immersion objective unless it is in contact with the oil.