1. Recombinant DNA Technology Bacteria, Yeast, & Virus Plasmids

2. What is a Plasmid? Circular DNA found in bacteria, viruses, and yeast

3. Recombinant Plasmids Plasmids that have foreign DNA inserted into them in a process called Recombinant DNA Technology

4. Methods of Recombinant Technology Transformation – Plasmid and foreign DNA are cleaved by restriction enzymes – Uses DNA ligase to insert DNA into vector – Vector inserted into host cell (usually E. coli)

5. Methods of Recombinant Technology Non-Bacterial Transformation – Similar to transformation except E. coli/bacteria is not used as a host cell – DNA is injected directly into the nucleus of the cell being transformed OR – The host cells are bombarded with high velocity microprojectiles such as gold or tungsten coated with DNA

6. Methods of Recombinant Technology Phage Introduction – Process of transfection (equivalent to transformation) except a phage is used instead of bacteria – Lambda or M13 phages are used to produce phage plaques which contain recombinants

7. Antibiotic Resistance The Evolutionary Arms Race http://www.youtube.com/watch?v=9CuhqQzBACQhttp://www.youtube.com/watch?v=9CuhqQzBACQ (Ep1) http://www.youtube.com/watch?v=2L82V6VPJkQhttp://www.youtube.com/watch?v=2L82V6VPJkQ (Ep2) http://www.youtube.com/watch?v=D0_FTJnhzXAhttp://www.youtube.com/watch?v=D0_FTJnhzXA (Ep3) http://www.youtube.com/watch?v=ofL52tWhacYhttp://www.youtube.com/watch?v=ofL52tWhacY (Ep4) http://www.youtube.com/watch?v=Chmb2g7atZ4http://www.youtube.com/watch?v=Chmb2g7atZ4 (Ep5) http://www.youtube.com/watch?v=J-VOvideRZAhttp://www.youtube.com/watch?v=J-VOvideRZA (Ep6)