1. Automated Gating of Flow Cytometry Data using Rho Path Distance http://www.genengnews.com/insight-and-intelligenceand153/flow-cytometry-goes-atomic/77899583/ Keyi Wu Project Leader: Matthew Hirn

2. Flow Cytometry Laser based Used in: cell counting cell sorting biomarker detection indicates normal biological & pathogenic processes protein engineering Physical & chemical properties of cells Diagnosis of health disorders http://www.scq.ubc.ca/flow-cytometry-a-technology-to-count-and-sort-cells/

3. Gating Sequentially separate regions based on fluorescence intensity Specific protocol exists for diagnostic and clinical purposes

4. Path Distance

5. rho -> 0 : shortest path measured purely based on adding local distance rho -> Inf : distance defined by “worst” segment in path

7. Compute in Parallel Divide data (size N) into sections each with less than a desired amount of points (n) Define boundary points for all boxes Calculate local and global distance within each box & for boundary points Find path between points not in same box through boundary paths

8. Principal Component Analysis (PCA) Linear dimensionality reduction technique Finds dimension in which variance is maximal

9. Partitioning Data BoxesPCA

11. Ratio of distance computed from parallel vs. regular

12. Future work Increase efficiency of code FlowCAP - Flow Cytometry: Critical Assessment of Population Identification Methods