1. Strawberry DNA Extraction

2. DNA: Basics Deoxyribonucleic acid Genetic material ◦ Chain of molecules linked together ◦ DNA contains instructions for traits Wrapped tightly around “histones” Forms chromosomes Housed in nucleus

3. DNA: Basics

4. DNA: Structure Double-helix (twisted ladder) Sugar and phosphate backbone Nitrogen bases Subunit: “nucleotide”

5. DNA: Replication Special pairing between nitrogen bases New strand is copy of old strand

6. DNA Extraction: Step 1 Break the cells open (using force)

7. DNA Extraction: Step 2 Dissolve the cell membrane and nuclear envelope (using detergent/soap) Current Protocols in Protein Science Published by John Wiley & Sons, Inc.

8. DNA Extraction: Step 3 Remove proteins that are bound to DNA (using salt to dissolve them)

9. DNA Extraction: Step 4 Precipitate DNA (with ethanol)

10. DNA Extraction: Why? Species identification VS.

11. DNA Extraction: Flow chart