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Today: DNA Extraction! Shireen Rudina
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Procedure Mash up your fruit with mortar and pestle, it must be completely mashed before #2. Filter the solution into a beaker, and then transfer to a test tube. Centrifuge for 10 minutes Make a solution with 10 mL of detergent and 4 g of salt. Add this solution to your test tube, and swirl to mix. Incubate for 10 minutes. Add COLD ethanol to the test tube and watch your chromatin form!
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Centrifuge must be balanced
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What are some of the barriers we need to break to extract DNA?
Cell wall Ribosome Golgi complex Nuclear membrane Cell membrane
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Which of these objects does NOT have DNA?
Humans= 30,000 genes 3,000 genes 20,000 Fish Rock Flower Bacteria (prokaryotic)
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