1. PigmentPigment Why does this bacteria change color?

2. Garcia, Angela Urbina, Maria 2 Who?Who? Angela Garcia (right) Maria Urbina (left) Northeast Magnet High School Bio-Research Club 2006-2007 Juniors

3. Garcia, Angela Urbina, Maria 3 ExperimentsExperiments Experiment # 1 Salinity Concentration –To what extent does the salt concentration affect marinococcus coloration? Experiment # 2 Darkness –To what extent does the light in the room affect marinococcus coloration? Experiment # 3 Temperature (°C) –To what extent does the temperature affect marinococcus coloration? Experiment # 4 pH level –To what extent does the pH level affect marinococcus coloration?

4. Garcia, Angela Urbina, Maria 4 MarinococcusMarinococcus Flavin pigment –(natural yellow pigment) Halophilic –(grows only in salt rich environment) Gram positive cocci –(Spherical, spiral, or curved shaped bacteria that stained purple/blue during iodine staining)

5. Garcia, Angela Urbina, Maria 5 Items beingtestedItems beingtested Marinococcus –# 31 (light yellow) –# 32 (bright orange) Rate at which Growth can be Achieved –1% - 20% Salt Content –4° - 50° C –pH 9 - pH 11 Control –37°C –pH 9 –10% Salt Concentration

6. Garcia, Angela Urbina, Maria 6 MarinococcusMarinococcus Our bacteria, here you can see the difference in the colors.

7. Garcia, Angela Urbina, Maria 7 Ex: 1 Salinity Concentration To want extent does salinity concentration affect coloration of marinococcus? –H 1 If salt content is increased then the coloration of marinococcus will be affected. –H 0 If salt content is increased then the coloration of marinococcus will not be affected. –H 2 If salt content is decreased then the coloration of marinococcus will be affected.

8. Garcia, Angela Urbina, Maria 8 The TestThe Test Testing –Minimum 1% salinity –5% salinity –Optimum 10% salinity –Maximum 20% salinity Control –Temperature - 37° C –pH level - 9 Amount Tested –24 petri dishes will be set up. 12 for each type, #31 & #32, of the bacteria.

9. Garcia, Angela Urbina, Maria 9 Preliminary DataPreliminary Data 2-8-07 –Started Colonies on 1%,5%,10%, and 20% agar plates. Also began new colonies and placed the older ones in freezer to prevent further growth.

10. Garcia, Angela Urbina, Maria 10 Collecting bacteria from the original slants. Proper technique for creating individual colonies. Preparing to flame loop for transfer. Fun in the lab.

11. Garcia, Angela Urbina, Maria 11 First Week Results 2-15-07 #31 –1% - lead sized individual colonies with diminutive, small, growth –5% - lumped colonies in a strip line but no individual growth 2-15-07 #32 –1% - diminutive growth better than the 20% –5% - an abundant of tiny colonies sparsely spread

12. Garcia, Angela Urbina, Maria 12 2-15-07 #31 –10% - As expected, colonies grew quite well, many useable individual colonies –20% - One mass colony, most likely the first streak when transferred, no individual colonies 2-15-07 #32 –10% - As expected, colonies grew quite well, many useable individual colonies –20% - Two mass colonies, most likely the first streak when transferred, no individual colonies First Week Results

13. Garcia, Angela Urbina, Maria 13 Other Notes 2-15-07 –The temperature had settled to 30°C on 2-15-07 so we moved it up to the control 37°C. –Conclusion after first week The salinity concentration has no affect on coloration, just on the growth of marinococcus.

14. Garcia, Angela Urbina, Maria 14 ImagesImages 2-21-07 2-22-07

15. Garcia, Angela Urbina, Maria 15 ImagesImages 2-21-07

16. Garcia, Angela Urbina, Maria 16 ImagesImages 2-21-07

17. Garcia, Angela Urbina, Maria 17 ImagesImages 2-21-07

18. Garcia, Angela Urbina, Maria 18 Other Notes 2-21-07 –Temperature was back at 30°C, moved up to the control –No useable image of 1% #31 was taken so 2-22-07 another picture will be collected –Agar has dried out, crystals have formed on most plates

19. Garcia, Angela Urbina, Maria 19 Ex : 2 Darkness To want extent does the light in the room affect coloration of marinococcus? –H 1 If colonies of the marinococcus are kept in the dark then coloration will be affected. –H 0 If colonies of the marinococcus are kept in the dark then coloration will not be affected. –H 2 If colonies of the marinococcus are kept in the light then coloration will not be affected.

20. Garcia, Angela Urbina, Maria 20 The TestThe Test Testing –Dark (dark box) –Light (Florescent light) Control –Salt Concentration - 10% –Temperature - 37° C –pH level - 9 Amount Tested –6 petri dishes will be set up. 3 for each type, #31 & #32, of the bacteria.