1. Production of Erythropoietin in Pichia Pastoris Eporis – Team 15 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

2. Team Eporis 1/10 Abby Leistra Stephen Tubergen Zion LeeNick Giles StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

3. Background: What is Erythropoietin? Erythropoietin (EPO) is a glycoprotein hormone Naturally made in kidneys Promotes red blood cell production Treats anemia caused by Chronic kidney disease Cancer chemotherapy HIV therapy $4 Billion US Market in 2013 glycam.org 2/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

4. Problem Chinese Hamster Ovary (CHO) Cells Slow-growing Many purification steps nikon2.magnet.fsu.edu Solution 2013.igem.org Pichia Pastoris Cells Fast-growing Easy purification of protein 3/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

5. EPO Structure 4/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps Nett, J. H.; Gomathinayagam, S.; et. al. J. Biotechnol. 2011, 157, 198-206. Polyethylene Glycol (PEG) Large Negatively charged

6. Capacity Estimate How much? How big? Depends on: Market Volume Yeast Productivity Separation Recovery en.wikipedia.org Bottom Line: 169 g EPO/yr → 8500 L/yr 5/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

7. Process Model -PFD 6/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps http://www.esslab.com/Hamilton/sensors/sensor_applications/bio.html

8. Process Model -Bioreactor Key Considerations Oxygen Distribution Impeller Shear Force 7/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps http://2010.igem.org/File:UCL-BIOREACTSRS.png.

9. Process Model - Filtration Ultrafiltration Microfiltration Houp, R. C. J. Validation Tech. 2009, 40-45. 8/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

10. Business Model Three different alternatives Start –up Contracting Leasing 9/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps stcaustralia.org

11. Future Steps Provide detail to the process model Calculating flow rates in and out of major process units Determining process subunits Evaluate alternatives Business model Process units Optimize selected process model 10/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

12. Questions?