1. Revision!Revision! L.O: to summarise biochemical tests to apply knowledge to questions

2. Biochemical Tests are used to indicate the presence / absence of biological molecules… http://www.bbc.co.uk/schools/gcsebitesize/science/add_oc r/chemicals/biologicalcompoundsact.shtmlhttp://www.bbc.co.uk/schools/gcsebitesize/science/add_oc r/chemicals/biologicalcompoundsact.shtml

3. Summary Table Biological Molecule Biochemical test Positive Result

4. Summary Table Biological Molecule Biochemical test Positive Result Protein Lipid Reducing Sugar Non Reducing Sugar

5. Summary Table Biological Molecule Biochemical testPositive Result ProteinBiuretPurple Lipid Reducing Sugar Non Reducing Sugar

6. Summary Table Biological Molecule Biochemical testPositive Result ProteinBiuretPurple LipidEmulsion Test (ethanol + H 2 O) White precipitate Reducing Sugar Non Reducing Sugar

7. Summary Table Biological Molecule Biochemical testPositive Result ProteinBiuretPurple LipidEmulsion Test (ethanol + H 2 O) White precipitate Reducing Sugar Benedicts + HEAT Brick red Non Reducing Sugar

8. Biological Molecule Biochemical testPositive Result ProteinBiuretPurple LipidEmulsion Test (ethanol + H 2 O) White precipitate Reducing Sugars (mono and most di) Benedicts + HEATBrick red Non Reducing Sugar (sucrose) 1. Hydrolyse into monomers by HCL + boiling. 2. Neutralise with alkali. 3. Retest with Benedicts + HEAT Brick red

9. Application of Knowledge http://www.classtools.net/education-games-php/timer

10. Question 1 a)A = Protein B = Lipid C = Reducing Sugar b)Hydrolyse with acid and then neutralise with alkali, re test with Benedicts c)Carbon, Hydrogen, Oxygen

11. Question 1 (again!) 1 a) C 12 H 22 O 11 (Because H2O is formed in the condensation reaction) 1 bi) Purple 1 bii) Because it’s a protein 1 c) Benedicts AND heat Brick Red

12. Application of Knowledge http://www.classtools.net/education-games-php/timer

13. Question 1 (again!!) 1 a) Nitrogen 1 b i) Benedicts AND heat Brick Red 1 b ii ) Substance A (glucose) 1 c i) Forming bonds, with the removal of water 1 c ii) C (protein) 1 d i) Substance D 1 d ii) Maximum amount of hydrogen atoms NO double bonds / all carbons attached to two hydrogens