1. Case Study 3: Werner’s Syndrome a progeriac disease Cell Division Cell Cycle Aging What mechanisms control the proliferation of cells? What governs the life span of an organism? Cell death as a necessary and important part of development: Apoptosis (programmed cell death, pcd)

2. Context: George Martin, 1978 ‘Genetic Syndromes in Man with Potential Relevance to the Pathology of Aging’ < 7000 genes: involved in degenerative processes associated with aging Between 70 and 7 genes: control processes having large impact on senescence What is cell senescence? Divide certain # of times then enter G 0 and eventually die

3. Aging a multigene process 10 genetic diseases that mimic aging process—but only in part Chromosomal aneuploides  Down’s syndrome Known single mutant gene  Werner’s syndrome Unknown but thought to be single gene  Hutchinson-Gilford’s Progeria Dementia, cataracts, diabetes, hair graying, cancer Skin ‘thinning’, Hair graying and loss, atherosclerosis, Cataracts, cancer diabetes, osteoporosis Skin ‘thinning’ hair loss, atherosclerosis, osteoporosis, hypertension Birth In 20’s Helicase Birth/1yr ?? Appears

4. www.pathology.washington.edu/werner/ Werner’s Syndrome

5. Werner’s history Named for C. W. Otto Werner (1879-1936) Rural doctor, medical officer in German Navy WWI Rare autosomal recessive disease Approx 1 in 200 people carriers for defective gene Approx 3 in 1,000,000 people have the disease (Slightly higher percentage in Japan) Onset of symptoms early to mid 20’s, Major cause of death—heart attack in mid 40s

6. Cells of Progeria and WS Cell Culture What do cells need to proliferate? Progeriac Fibroblasts: Rarely ever double Few cell generations before death When compare fibroblasts of child with Progeria and their parent Child’s cells are ‘older’ in terms of replication Fibroblasts ‘normal cells’ divide ~12 to 24 hours Divide approx 50times in culture Note: Often ‘Progeriac’ used to describe any premature aging as well as the specific disorder Hutchinson Gilford Progeria

7. Why do we age?/How how do we age? Short answer: Don’t know 3 Hypotheses for Aging: The 3 R’s: Mutation effecting DNA reading, replicating or repair 1) Free Radical Theory: Aging due to accumulation of damage from free radicals 3) Helicase defect: Mutation Chromosome 8 in WRN gene all 35 known mutations result in truncated protein all ‘remove’ nuclear targeting sequence different mut’s associated with different cancers 2) Telomere Theory: Chromosome ends shorten with divisions Cause of Werner’s syndrome

8. Case Study Focuses Cell Cycle and its regulation Telomeres and replication Cell death: damage and apoptosis

9. Lab to wrap up by end of this week No new setup after Thursday so all data in by Sunday REVISED DEADLINES: Stretched and divided No flow chart turned in (although still a good idea to make one) Before Nov 21 st 5pm: (Tuesday) Half of group posts Intro, Results References, other half posts Results, Discussion, References (see table to find out what you are writing and instructions for every section) Before beginning of your lab on week of Nov 27th Group members print specific section sections and revise. Keep the version with comments on it, post the revised version. You will have time to meet as a group during the lab period (see table to see what you are revising and instructions for reviewing/revising) Before December 5 th at noon (Tuesday) Groups meet and develop one article containing Title, Abstract, Intro, Results Discussion, Experimental Procedure and References. Each person’s version with comments and the final group article is submitted as a portfolio for evaluation. The drafts and comments will be indications of level of participation. I will evaluate the final product.

10. Therefore Each PERSON posts 1/4 draft they wrote edits a draft and turns in the copy they wrote on posts 1/4 draft another wrote and they edited/revised Each GROUP turns in a full lab article (not ea. person) hard copy Whole group follows process that is more similar to published article writing (and is done in more manageable pieces)