1. Copyright © Dean Madden, 2013

2. The bacterium Bacterial chromosome Plasmids Cytoplasm Ribosomes
Cell wall
Cell membranes

3. The plasmid
Origin of replication this is needed so that the plasmid can be copied, using the bacterium’s DNA copying machinery
Kanamycin resistance gene the enzyme encoded by this gene stops the antibiotic kanamycin from working
GFP gene This gene encodes green fluorescent protein, which glows in UV light

4. Aequorea victoria

5. GFP

6. Glo Fish

10. These should be grown 1–2 days in advance
Stock bacteria

11. Twiddle vigorously to dislodge the bacteria and disperse them
Transformation buffer

12. Leave on ice for 15 minutes to recover

13. Bacterial cell with pores in cell membrane
GFP
plasmid
DNA
Bacterial cell with pores in cell membrane

14. 3 drops
(100 µL)
LB agar
+ kanamycin
+ IPTG

16. Seal the plates with tape
Label the base of each plate with your initials, the date and the type of bacteria used
Seal the plates with tape

22. TRANSFORMED CELLS MUST BE DESTROYED AFTER USE
BIOHAZARD
TRANSFORMED CELLS MUST BE DESTROYED AFTER USE