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Mohammad Emaneini, PhD Assistant Professor in Microbiology Department of Microbiology School of Medicine Tehran University.

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Presentation on theme: "Mohammad Emaneini, PhD Assistant Professor in Microbiology Department of Microbiology School of Medicine Tehran University."— Presentation transcript:

1 http://tums.ac.ir/faculties/emaneini Mohammad Emaneini, PhD Assistant Professor in Microbiology Department of Microbiology School of Medicine Tehran University of Medical Sciences emaneini@tums.ac.ir

2 Emaneini M. PhD. Transformation A DNA fragments/plasmids of donor bacteria Taken up & incorporated into the genome of recipient cell Natural competence Streptococcus pneumoniae, Haemophilus influenzae, Neisseria gonorrhoea Induced competence Cold CaCl 2 & heat shock: E. coli Protoplast: Bacillus, Streptomyces Electroporation: a brief pulse of high-voltage electricity

3 Emaneini M. PhD. Transduction Transfer of DNA by Bacteriophage via Lytic & Lysogenic cycle Generalized transductionSpecialised transduction

4 Emaneini M. PhD. Discovered 1946 Lederberg & Tatum Donor: Containing conjugative plasmid (F+) Recipient: Bacterial receiving the plasmid (F-) Conjugation High-frequency recombination (Hfr)

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