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Published byIsabel Stevens Modified over 9 years ago
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M.E. Wilson Pig Reproduction Pan Pacific Pork Expo 2006
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30 Pigs weaned/ sow/ year
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Economics of reproduction 3-3.5 US cents per market pig/EBV point above the average $2.35 improvement in each generation
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5 Natural Mating Natural Service vs Artificial Insemination
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Natural Service Artificial Insemination Total sperm cells 30-80 billion3 billion Total volume200 – 400 ml75-85 ml Seminal Plasma/ insemination 160-350 ml8-12 ml
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Boar IDVolume% viable cellsMean recovery 730775.352% 1228670.624 % 1432466.635% 161994720% 1723483.639% Lebowa and Wilson, unpublished data, 2005 Which Boar will have the Highest Fertility?
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Mean Fertility Results Boar IDservicesFarrowing %Total born 7309313.18 12298312.74 1431459.22 162710012.04 1727526.5 Lebowa and Wilson, unpublished data, 2005
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Single Boar 93 79.6 9.26 Two different Boars 106 89.6 10.68 Number of Matings % conception Litter Size Thacker and Kirkwood, 1988 Effects of heterospermic matings verses homospermic matings on litter size
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Comparison of Breeding Methods United Feeds, 1998 Method Number of Litters Farrowing rate % Total born - mummy AI- 2 different boars 2208812.36 Combo - 1 natural 1AI 2188812.06 Natural serv1898811.9 AI- 1boar1858811.62 A A A B Different superscripts P<.05 B B
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Ultrasonography Monitoring Ovarian Activity No Follicles Follicles Kemp and Soede, 1996 follicles
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Interval between insemination and ovulation Sows with >90% Normal Embryos Fertilization % Insemination48-401729 Pre-ovulation40-321437 32-2447 24-1679 16-88394 8-08693 Ovulation0-85475 Post-ovulation8-165362 The effects of the interval between insemination and ovulation on Fertilization rate and embryo viability (Soede, et.al., 1995a)
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Synchronized Estrus and ovulation 500 – 750 IU of eCG Pregnecol (Canada) 400 IU eCG and 200 IU hCG (PG 600) 42 hours after hCG ovulation typically occurs 36-38 hours after pLH ovulation will be induced
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Billions sperm cells/dose Number of doses 330 2.536 245 1.560 IUI -.6150 Managing dose potential per boar
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The issue is not TECHNOLOGY The issue is IMPLEMENTATION
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Transcervical Deep Uterine Artificial Insemination Traditional
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TreatmentNTotal Pigs+/- Control Conventional 4 billion 100947- IUI 4 billion1001086+ 139 IUI 1 billion100892- 55 IUI.5 billion100712- 235 The number of pigs per 100 matings with 0.5, 1 or 4 billion cells transcervical 1 or 4 billion cells intra cervical 1 Transcervical catheter passage was 94%. Hemorrhaging observed on Catheter 4% of matings (n=620) K. Rozeboom
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Sexed Sperm Cells Non-surgical implantation of embryos Frozen Semen Expanding genetic potential and reducing variation
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Let’s jump into the future
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Sperm sexing technology Based on the fact that more (2.8% – 7%) DNA is contained in X-bearing than in Y- bearing sperm
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Surgically Flushing Embryos
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Midwest Commercial ET Project Embryo Survival to Birth from Farrowed Sows Piglet Genotype Total Born Embryos Transferred (farrowed sows only) Pigs per Embryo Duroc182375.49 White66103.64 Total248478.52
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Litter of 14 pigs from non-surgical transfer 15 embryos transferred 14 pigs born alive
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Cloning Dolly, the sheep (1996) First mammal to be cloned from adult cells (mammary) Dolly Surrogate mother
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Viable cloned offspring (litter size ave. = 4-8) Adult donor Cloned embryos Transferred within 24 hrs - Porcine Surgical transfer into oviduct- 50-100 clones Chromatin Transfer
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Benefits of Cloning Advance superior genetics Feed efficiency Rate of gain Carcass traits Gene markers for disease Research model Removes more variation Medical models Testing human diseases
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