1. Cell transportation

2. TWO MAJOR TYPES OF TRANSPORT THROUGH BIOLOGICAL MEMBRANE ● Transport across biological membrane Transport across, but not through, membrane

3. 1.Endocytosis 1.1. Phagocytosis : solid particle, “ cell eating ” 1.2. Pinocytosis : s oluble molecules “ cell drinking ” 1.3. Receptor-mediated endocytosis 2. Exocytosis Transport across, but not through, membrane = Bulk transport

5. 1.3. Receptor-medated endocytosis SUTHILUK PATUMRAJ, Ph.D. Department of Physiology Faculty of Medicine, Chulalongkorn University LDL-c

6. 2. Exocytosis Example: Neurotransmitters ; acetylcholine (Ach) SUTHILUK PATUMRAJ, Ph.D. Department of Physiology Faculty of Medicine, Chulalongkorn University active zone trafficking

7. 1.SIMPLE DIFFUSION - osmosis : solvent - diffusion : solute 2. CARRIER MEDIATED TRANSPORT 2.1 FACILITATED TRANSPORT 2.2 ACTIVE TRANSPORT 2.2.1 PRIMARY ACTIVE TRANSPORT 2.2.2 SECONDARY ACTIVE TRANSPORT Transport across biological membrane

8. Membrane Transport of Small Molecules

9. FICK FIRST LAW OF DIFFUSION J = - D A dC / d X D = DIFUSSION COEFFICIENT A = AREA dC = CONCENTRATION GRADIENT dx = DISTANCE Lipid solubility size viscosity temperature SIMPLE DIFFUSION

10. OSMOSIS About 300 mlosmole/L Hemolysis crenation

11. IONIC CHANNEL GATING MECHANISMS SELECTIVITY

12. CARRIER MEDIATED TRANSPORT SUTHILUK PATUMRAJ, Ph.D. Department of Physiology Faculty of Medicine, Chulalongkorn University Competition Molecules X + Y [ C ] ECF [ C ] ICF

13. GLUCOSE TRANSPORTER AMINO ACID TRANSPORTER FACILITATED TRANSPORT

14. ACTIVE TRANSPORT PRIMARY ACTIVE TRANSPORT Na + - K + ATPase K + - H + ATPase Ca 2+ - ATPase SECONDARY ACTIVE TRANSPORT CO-TRANSPORT COUNTER-TRANSPORT ATP Na+ Energy source

15. SUTHILUK PATUMRAJ, Ph.D. Department of Physiology Faculty of Medicine, Chulalongkorn University “ Homeostasis ”