1. QoD (HB) 5-31-11  Do you remember what a plasmid is?

2. Plan of the Day (HB)  How engineering bacteria works  Overview and points to consider for pGLO bacterial transformation lab tomorrow  Presentations

3. How can bacteria exchange genes?  Through tunnel like connections  Viruses carry bacterial genes  Bacteria take up DNA from surrounding environment

5. Recombinant DNA Technology Some Bacteria are used to mass produce specific desirable genes and proteins

6. Cutting and Pasting DNA

7. Restriction enzymes cut DNA molecules at specific locations Fragments of DNA spliced together from two different sources produces a recombinant DNA molecule

8. Cloning of a human gene for a hypothetical protein V

9. Applications of recombinant DNA technology Bacteria engineered to clean up toxic waste Mass-production of useful chemicals Mass production of proteins and hormones for medical uses Develop vaccines against disease-causing microbes Develop genetically modified multicellular organisms

10.  pGLO bacterial transformation lab preparation  http://biologywithsaxe.blogspot.com/20 09/01/lab-9-bacterial-transformation- using.html http://biologywithsaxe.blogspot.com/20 09/01/lab-9-bacterial-transformation- using.html

11. Allows bacteria to survive in the presence of ampicillin In the presence of arabinose produces a protein that will turn on the GFP gene When turned on will produce a fluorescent green protein that will glow under UV light