1. BIOLASE THE INNOVATIVE, RESEARCHED AND PATENTED IMMUNO-MODULATOR FOR ALL MONOGASTRICS

2. BIOLASE THE SYMBIOTIC

3. INTESTINAL MICROBIAL FLORA (MONO GASTRIC) GRAM POSITIVES STREPTOCOCCUS LACTOBACILLUS BIFIDOBACTERIUM EUBACTERIUM PEPTO-STREPTOCOCCUS GRAM NEGATIVES CLOSTRIDIUM VEILLONELLA ENTEROBACTERIACEE YEASTS (less than 0.1%)

4. STREPTOCOCCUS faecium LACTOBACILLUS acidophilus, fermentum, salivarius, animalis, reuteri BIFIDOBACTERIUM Globosum, pseudologum, animalis, thermophilum, boum, magnum, pullorum, suis, cuniculi, choerium. (Sneath P.H.A., et all. 1986)

5. INTESTINAL MICROBIAL FLORA INTERACTION MICROORGANISM / SURFACE INTERACTION MICROORGANISM/ RESISTANCE 2 PRIMARY FUNCTIONS:

6. Interaction MICROORGANISM/ SURFACE A.PROTEOLYTIC ACTION B.UROLYTIC ACTION

7. Interaction MICROORGANISM/RESISTANCE A.ORGANIC ACID PRODUCTION (Acetic and Lactic, mostly) -Antibacterial effect -Lower ammonia absorption -Lower amines absorption

8. Interaction MICROORGANISM/RESISTANCE B.Immunological activation C.Production of Vitamins and Enzymes D.N-nitrosamines Degradation E.Reduction of Ammonia and Hydrogen Sulphide. F.Production of bactericidal substances (self regulation)

9. INTESTINAL ECO-SYSTEM STABILITY BALANCE

10. LIVE LACTOBACILLI/STREPTOCOCCI ADMINISTRATION Sensitive to chemical & physical stress (heat, pH,…) Slow growth (6-8 hours) BIO-TYPE BIFIDOBACTERIUM ORAL ADMINISTRTATION IMPOSSIBLE: ANAEROBES

11. LIVE YEASTS Naturally: Acid resistant pH resistant Antibiotic resistant

12. LIVE YEAST LIVE ORGANISM (ANIMAL) -CONSUMES -PRODUCES CARBOHYDRATES / SUGARS ALCOHOL – CARBON DIOXIDE (BREWERY) ( BAKERY)

13. DEAD YEAST BY-PRODUCT OF BREWERY INDUSTRY SOURCE OF PROTEIN ALL NUTRIENTS ALREADY USED UP DOSAGE 5-10 Kg/Ton - DRIED-UP YEAST-

15. BIOLASE DOES NOT FERMENT DOES NOT REPRODUCE DOES NOT PRODUCE ALCOHOL DOES NOT PRODUCE CARBON DIOXIDE NO MORE ALIVE THE CELL IS WHOLE VITAMINS ENZYMES NUCLEOSIDES NUCLEOTIDES

16. BIOLASE cells at electronic microscope

18. BIOLASE cells after spray-drying

19. BIOLASE BIO-ACTIVITY CAN BE MEASURED CAPACITY TO MAKE A LACTOBACILLI&BIFIDA POPULATION GROW MORE THAN A CONTROL GROUP BIOLASE BIO-ACTIVITY: MIN. 4.5

20. IN VITRO BIO-ACTIVITY TEST (INCUBATION TIME: 8 hours – temperature: 37°C) GROWTH

21. BIOLASE CONTROL 3124567 0 10 20 30 Growth curves of 2 lactobacilli coltures, incubated at 37°C. After 5 hours you have BIOLASE highest bioactivity, as expression of the ratio between the two coltures growth levels (measured as Optical Density) INCUBATION (HOURS) OPTICAL DENSITY BIOLASE BIOACTIVITY

22. Bifida BIOLASE cells “digesting”

23. Lactobacilli a BIOLASE cell “ eating ”

24. Bifida BIOLASE cells “digesting”

25. Tadpoles The one on the right has been fed BIOLASE

26. Lactobacilli Population (white) attacked by pathogenes (black)

27. BIOLASE Cells(brown) super feeding Lactobacilli&Bifida

28. Lactobacilli&Bifida population has grown stronger and larger, thanks to BIOLASE

29. BIOLASE is the ideal nutrient for Lactobacilli&Bifida growth