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Introduction to Adenoviruses

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Presentation on theme: "Introduction to Adenoviruses"— Presentation transcript:

1 Introduction to Adenoviruses
Hugh Fackrell University of Windsor Windsor, ON Canada

2 Adenoviruses Outline General Properties of Adenovirus
Clinical Diseases Transmission Diagnosis Prevention and Control Baron’s Web Site

3 Adenovirus Structure isocahedral - 70-100 nm 252 capsomers
240 hexons 12 fibrillar pentons dsDNA linear 2 major core proteins 2 minor proteins

4 Adenovirus Core Proteins
Protein V- histone like Protein VII -bridge to capsid Protein mu-?? Terminal Protein covalently linked to DNA circularizes DNA primer for DNA replication

5 Model of Adenovirus

6 Adenovirus virions

7 Adenovirus replication

8 Adenovirus mRNA Adeno DNA reaches nucleus
terminal protein binds to host DNA Viral Genes expressed Adenovirus mRNA moves to cytoplasm 13S mRNA & 12S mRNA have introns Intron splicing of mRNA gives several different proteins

9 Clinical Manifestations
Conjunctivitis acute folicular conjunctivitis epidemic keratoconjunctivitis Pharyngitis- acute febrile Pneumonia- sequalae Cystitis Infantile Gastroenteritis

10 Host Defenses Neutralizing antibodies -teenagers
Cytotoxic T lymphocytes Interferon

11 Epidemiology Children Military recruits Immunocompromised patients
6 months-2 years highly susceptible extends to 5-9 years of age types 2,1,3,5,7 type 1 & 2 causes 60% of infections Military recruits Immunocompromised patients

12 Antigenic types Mastadenovirus Aviadenovirus
47 types of human adenovirus Aviadenovirus 80 types infect birds No common antigen for all adenoviruses some cross reaction with hexon protein

13 Human Adenovirus Types (Mastadenovirus H)
Subgenus Serotype A ,18,31 B ,7,11,14,16,21,34,35 C ,2,5,6 D ,13,15,17,20,22-30, 32,33,36-39 E F ,41

14 Pathogenesis Droplet transmission Respiratory or ocular secretions
Persistent infection in tonsils

15 Adenovirus causes epidemics of acute respiratory disease in military recruits

16 Vaccination limited application because adenovirus is associated with tumorigensis in animals rarely serious complication attenuated vaccine to 4,7,21 applied to military recruits

17 Adenovirus-Host Cell Interactions
Viral latency human- tonsils Abortive infection-hamster or monkey cells Semi permissive infection- rat cells Productive infection-human cells Malignant transformation- rat cell lines Tumor induction- hamsters First time for Human virus (Type 12)

18 Adenovirus induced tumour in hamster

19 Adeno Associated Satellite Virus

20 Diagnosis Clinical manifestations
Rise in specific antibody titer between acute phase and convalescent phase Isolation of virus by tissue culture

21 Cytopathic Effect diploid cells, early cpe-125x
Apoptosis induced by E1A proteins made from 12S & 13S mRNA

22 Cytopathic effect on primary rhesus monkey kidney cells
<<early late>>

23 Adenovirus pneumonitis
bronchial occlusion

24 Adenovirus inclusions
Basophilic nuclear inclusions in lung alveoli

25 Nuclear inclusions of Adenovirus

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