1. Biofilms

2. Dental plaque

3. Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants 4- Associated diseases

4. Tubing obstruction Equipamiento dental >30años

5. MS 2- M+M+

6. Biofilm formation on a voice implant

7. Contact lens

8. Catheter

9. P. Aeruginosa biofilm in lung

12. Structure

15. Biofilm formation process

16. Glass surface in the lab

19. MBFHBFLBFWT Citrate Glucose+Caa Genetic analysis of biofilm formation

20. Genetic characterization of biofilm formation - Requirement of movement - Adhesins -Signaling -Glycocalyx formation

21. Movement requirement -Flagelar-mediated mobility -Non-motile mutants in P. Fluorescens -Twitching -Mutants in type IV pili in P. aeruginosa -Suppression by cells “stickier” -ompR and adhesin Curli in E. coli

22. Adhesins -Type I pilus (fimH) in E. coli -Type IV (MSHA) pilus in V. cholerae -Curli adhesin

23. Signaling -Quorum sensing

25. QUORUM SENSING:DENSITY-DEPENDENT EXPRESSION CULTURE DENSITY AUTOINDUCER TRESHOLD

26. GRAM-NEGATIVE COMMUNICATION: THE LuxI/LuxR LANGUAGE LuxI LuxR Target gene

28. GRAM-NEGATIVE COMMUNICATION: THE LuxI/LuxR LANGUAGE -Light production in Vibrio fisheri -Virulence in Pseudomonas aeruginosa (LasI/R, RhlI/R) -Opine formation in Agrobacterium tumefaciens (TraR/I)

29. GRAM-positive COMMUNICATION: THE PHEROMONE LANGUAGE ABC D Target gene Pheromone gene H

30. HYBRID LANGUAGES: THE CASE OF Vibrio harvey LuxO Target gene LuxN LuxLMLuxS LuxQ

31. -Competence for DNA uptake in Bacillus subtilis and Streptoccocus pneumoniae (ComX/ComP/ComA) -Sporulation in Bacillus subtilis -Conjugation in Enteroccus fecalis -Virulence in Staphylococus areus GRAM-positive COMMUNICATION: THE PHEROMONE LANGUAGE

32. QS-mutants

34. Glycocalyx formation -Alginate production in P. aeruginosa - EPS in V. cholerae (vps)

36. fimA lasI vps flagella rhl alg fimH Curli

37. Natural process of biofilm formation / Control by a blocking analogue