1. 1 Life Analytical Chemistry-Molecular Imaging (MI): Optical Imaging Gaolin Liang （梁高林）, Ph. D. Professor, Ph. D. Advisor Deptartment of Chemistry University of Science and Technology of China

2. 2 Optical Imaging Fluorescence imaging Non-Fluorescence- based optical imaging Microscopic fluorescence imaging Macroscopic fluorescence imaging bioluminescence imaging optical coherence tomography photoacoustic microscopy tissue spectroscopy

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4. 4 Fluorescence imaging: Microscopic fluorescence imaging History: In 1839, Rudolph Wagner visualized leukocytes rolling in blood vessels within membranous translucent tissues by using brightfield Transillumination. Nowadays: Several imaging approaches based on fluorescence microscopy that were established for visualizing cells in vitro have recently been adapted for in vivo imaging: multiphoton microscopy, laser-scanning confocal microscopy, fibre-optic approaches and spectrally encoded endoscopy.

5. 5 Fluorescence imaging: Macroscopic fluorescence imaging There are two main types of imaging approach: fluorescence reflectance and tomographic fluorescence. FMT-CT/FMT-CT fusion

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11. 11 Optical Imaging Fluorescence imaging Non-Fluorescence- based optical imaging Microscopic fluorescence imaging Macroscopic fluorescence imaging bioluminescence imaging optical coherence tomography photoacoustic microscopy tissue spectroscopy

12. 12 Non-Fluorescence-based optical imaging: bioluminescence imaging luciferase–luciferin pairs firefly (Photinus pyralis) luciferase– luciferin Renilla reniformis luciferase– coelenterazine Gaussia princeps luciferase– coelenterazine

13. 13 Non-Fluorescence-based optical imaging: bioluminescence imaging

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19. 19 Non-Fluorescence-based optical imaging: optical coherence tomography Optical coherence tomography is based on light scattering and can be used to image microscopic structures in vivo (at a resolution of 2–15 μm and to a depth of 1–3 mm)

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25. 25 Non-Fluorescence-based optical imaging: photoacoustic microscopy Photoacoustic microscopy uses short laser pulses to irradiate tissue and temporarily raise its temperature (by millikelvins). Thermo-elastic expansion then causes the emission of photoacoustic waves that can be measured by wide-band ultrasonic transducers, offering improved depth resolution in the 3–20 mm range

26. 26 Non-Fluorescence-based optical imaging: photoacoustic microscopy

27. 27 Non-Fluorescence-based optical imaging: photoacoustic microscopy

28. 28 Non-Fluorescence-based optical imaging: photoacoustic microscopy

29. 29 Non-Fluorescence-based optical imaging: photoacoustic microscopy

30. 30 Non-Fluorescence-based optical imaging: photoacoustic microscopy

31. 31 Non-Fluorescence-based optical imaging: tissue spectroscopy tissue spectroscopy detects relative changes in the way in which light interacts with tissue and has been used extensively to improve early detection of gastrointestinal malignancies

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