1. Detergent/Enzyme/Heat Break down the cell wall 60 o C breaks down nuclear membrane, but not DNA; 80 o C will break down DNA as well Chemistry of DNA extraction - some bio too!

2. DNA Extraction from Green Split Peas or raw wheat germ !

3. DNA Extraction Procedure - 1 Blend for 15 seconds: »DNA source – 100 ml green split peas »Cold water – 200 ml »Large pinch of table salt Pour mixture through a strainer Add detergent – 2 tablespoons Let sit for 5 – 10 minutes

4. DNA Extraction Procedure - 2 Pour mixture into test tubes – 1/3 full Add meat tenderizer – dip of wet stick Tilt test tube Slowly pour rubbing alcohol into the tube Equal amounts of alcohol & DNA mixture Watch DNA appear in the alcohol

10. DNA appears at alcohol/ mixture interface and can be spooled onto a stirring rod

11. Salt (Na +) is attracted to the phosphate group Denatures the protein that lyses (breaks down) DNA Alcohol Cold for two reasons: Slows down enzymes that break up DNA DNA is less soluble in cold temperatures

12. R-OH Water is HOH, like dissolves like Alcohol dissolves the water and pulls it away from the DNA R is shorthand for any organic group or hydrocarbon