1. Volunteer-based Stream Monitoring- Aquatic Macroinvertebrates Paul Steen Watershed Ecologist Huron River Watershed Council

2. Aquatic Insects are Diverse and Interesting!

3. 1. You never know what you will find! 3 Reasons to love aquatic bugs

4. 2. Crazy Camouflage!!

5. 3. Tough little buggers 3 Reasons to love aquatic bugs

6. Why collect bugs? Good indicators of localized conditions Diversity = Healthy stream Threats to bug diversity –Sedimentation –Habitat loss –Chemical pollution Easy sampling techniques- great for volunteers Generally abundant communities

7. Each team member gets a job- collector, scribe, picker, shuttler, leader, etc. Collector works upstream collecting along ~300 foot reach. Collector samples a variety of microhabitats. Store in 70% ethanol until identification. (avoid rubbing alcohol) Identification events How do we collect bugs?

8. No set time limit or bug number. This differs from DNRE Procedure 51. Some rules of thumb: -Sample until you feel like you do not see anything new, then go another 10 minutes. -Volunteers aren’t the best judge of this. -Generally, 30-45 minutes. -HRWC data- at least 50 creatures. How do we collect bugs?

9. Stream Macroinvertebrate Datasheet Collection Information Stream Conditions Habitats Sampled Identification Assessment

10. Classification System Kingdom- Animalia Phylum- Arthropoda, Mollusca, Annelida Class- Insecta (Crustacea, Pelecypoda, Gastropoda, Oligochaeta, Hirundea) Order- i.e. Diptera (true flies) Family- i.e. Tipulidae (crane flies) Genus- Species-

11. Eggs Flying Adults Aquatic Larvae (many growth instars) Midge (True Fly) Life Cycle Aquatic Pupae Complete Metamorphosis Other groups: Beetles Alderflies Dobsonflies Caddisflies Typically but not always terrestrial

12. Eggs Dragonfly Life Cycle Flying Adults Nymph (aquatic) Numerous growth instars Incomplete Metamorphosis Adults (typically but not always terrestrial ) Other groups: Mayflies Stoneflies True Bugs

13. Peckarsky, et al. Freshwater Macroinvertebrates of Northeastern North America. 1990. Wiggins. Larvae of the North America Caddisfly Genera (Trichoptera). 2 nd ed. 1998. Head Thorax Abdomen

15. Let’s meet the bugs! Group 1: Sensitive

16. Caddisflies Phylum: Arthropoda Class: Insecta Order: Trichoptera

17. Common Net-spinning Caddisfly NOT a sensitive species 10 mm Phylum: Arthropoda Class: Insecta Order: Trichoptera Family: Hydropsychidae

18. 3mm 44mm

19. Order Megaloptera: Note the Differences! Hellgrammite (family Corydalidae) No distinct, single tail Generally larger Alderfly (family Sialidae) Distinct, single tail Generally smaller Phylum: Arthropoda Class: Insecta Order: Megaloptera

20. Mayflies Phylum: Arthropoda Class: Insecta Order: Ephemeroptera

21. Gilled Snail Have an operculum or plate- like door that protects the opening of the shell and can be quickly closed to avoid predators. Coiled shells that usually open on the right-hand side. Phylum: Mollusca Class: Gastropoda Order: various

22. Nymph Adult Stonefly Phylum: Arthropoda Class: Insecta Order: Plecoptera

24. Water Penny Phylum: Arthropoda Class: Insecta Order: Coleoptera Family: Psephenidae

25. Watersnipe Fly Phylum: Arthropoda Class: Insecta Order: Diptera Family: Athericidae

26. Group 2: Somewhat-Sensitive

27. Order Megaloptera: Note the Differences! Hellgrammite (family Corydalidae) No distinct, single tail Generally larger Alderfly (family Sialidae) Distinct, single tail Generally smaller Phylum: Arthropoda Class: Insecta Order: Megaloptera

28. Beetles Phylum: Arthropoda Class: Insecta Order: Coleoptera

29. Beetles (order Coleoptera) Chewing or biting mouthparts 3 Pairs of legs Generally well sclerotized

30. Black Fly Phylum: Arthropoda Class: Insecta Order: Diptera Family: Simuliidae

31. Clams & other bivalves (phylum Mollusca, class Pelecypoda) Phylum: Mollusca Class: Pelecypoda Order: various

32. Crane Fly Huge Diversity in sizes (one useful character: mandibles on horizontal plane) Phylum: Arthropoda Class: Insecta Order: Diptera Family: Tipulidae

33. Crayfish Phylum: Arthropoda Class: Insecta Order: Decapoda

34. Dragonflies & Damselflies Stout body No tails Slender body Three tails Phylum: Arthropoda Class: Insecta Order: Odonata Suborder: Anisoptera & Zygoptera

35. Common Net-spinning Caddisfly NOT a sensitive species 10 mm Phylum: Arthropoda Class: Insecta Order: Trichoptera Family: Hydropsychidae

36. Scuds, Sideswimmers Phylum: Arthropoda Class: Crustacea Order: Amphipoda

37. Sowbugs Phylum: Arthropoda Class: Crustacea Order: Isopoda

38. Group 3: Tolerant

39. Aquatic Worms ( class Oligochaeta ) Note the segments! Phylum: Annelida Class: Oligochaeta Order: various

40. Leeches Phylum: Annelida Class: Hirundae Order: various

41. Midges Phylum: Arthropoda Class: Insecta Order: Diptera Family: Chironomidae

42. Miscellaneous Snails Do not have a plate-like covering over the shell opening. Has shell that spirals with opening usually on your left side, or shell that is coiled in one plane, or shell that is dome or hat shaped with no coils. Phylum: Mollusca Class: Gastropoda Order: various

43. True Bugs Tube-like sucking mouthparts Phylum: Arthropoda Class: Insecta Order: Hemiptera Wings hardened near the base and membranous everywhere else

44. Other True Flies Already seen… Phylum: Arthropoda Class: Insecta Order: Diptera