1. 2009 NMVB Conference NYU Dept of Cardiothoracic Surgery NYU Dept of Pathology Friday December 4 th, 2009

2. Personnel @ NYU Dept of Cardiothoracic Surgery: Harvey Pass, MD (surgeon, NYU steering committee member & PI) Audrey Sorensen, RN (research nurse coordinator) Samantha Lincoln (data manager) Ryan Harrington (lab technician, thoracic lab) Angela Pittman (research project coordinator) Dept of Pathology: Jonathan Melamed, MD (pathologist, NYU steering committee member) Jacinta Small (lab technician, pathology lab) Tian He (research coordinator; previously Timothy Jolis) Xiangtian Kong (pathologist, pathology review)

3. 2008-2009 Logistic improvements: Streamlined specimen/case-notification process Refined tissue procurement process with response team and procurement kits Synchronized local data with MVB database Progression on 5-year plans: Frozen TMA NYU MVB Paraffin TMA 1

4. Prospective cases: 63 Retrospective cases: 157 Total # of cases: 220 Year Retrospective Cases Prospective Cases Overall Total 2007 15731188 2008 021 2009 011 Total:220 Mesothelioma Accrual

5. Tissue Collected (research blocks) Collection Method# of Blocks / Samples of Frozen Tissue OCT124 FOIL130 Paraffin Blocks288 YearNew cases collected Collection Method Frozen tissue only Paraffin + Frozen tissue Paraffin tissue only 20087061 20099270 Emphasis on accruing extrapleural pneumonectomy cases Meso accrual data (cont’d)

6. Progression on 5 yr plans-Frozen TMA Frozen TMA Frozen Array mold kit

7. Frozen TMA Normal lung tissue Tumor tissue (mesothelioma) MVB #s indicate distinct patients N=13 pts mvb22 21 mvb21 89 mvb22 15 mvb22 19 mvb21 89 mvb22 15 mvb22 19 mvb21 90 mvb21 91 mvb21 97 mvb21 90 mvb21 91 mvb21 97 mvb22 17 mvb22 14 mvb21 99 mvb22 17 mvb22 14 mvb21 99 mvb22 03 mvb22 11 mvb22 13 mvb22 03 mvb22 11 mvb22 13 mvb22 12

8. Frozen TMA: Equipment & Accessories

9. NYU MVB Paraffin TMA 1 NYU MVB Paraffin TMA 1 Design Phase: N= cases/ Block X 4 B 4 TMA Blocks (same cases on each block) Control tissues, cell lines, frozen- to-formalin tissue (adv: standardized processing) Construction Phase: MTA II Microtomy: Limited number of sections; histocollimator Storage: In desiccators under nitrogen atmosphere High resolution scan of slide for website Plans for Paraffin TMA Sample paraffin TMA

10. Paraffin TMA: Equipment & Accessories Desiccators Tissue Micro-Arrayer

11. Proposed map for Paraffin TMA Cell Lines Tumor Control Liver Column → Row ↓012345678910111213 ALiver B C D E F G H I J K L M N O P Q R

12. Special Thanks to… Today’s conference attendees And the entire NMVB team