1. Rodent Breeding Colonies Bill Greer Penn State

2. The most Common Models  Colonies maintained by Animal Resources  Colonies are maintained by PI’s

3. OK, so What’s the Problem?   Numbers   Counting   Transferring   Strain Maintenance   Transgenic animals?

4. Problems – Ice Cream!!

5. How Many to Produce   How may the Animal Resource Program justify numbers?   How may the PI justify numbers? Maintain the strain Research needs Loss due to genotype Can only use one sex (M or F)

6. When do you count the animals? At   Birth   Weaning, or   Somewhere in between (e.g., first cage change)

7. Transferring from Breeding to Research Protocols? Counting against other protocols   Using it for research/teaching or testing? When to count Tracking the transfers

8. Any Guide Deviations? Maybe,   Single housing   Trio Breeding   Delayed cage changes

9. Transgenic?   Who’s making them – ARP, the PI, a service unit? IACUC and IBC Protocol   Any problematic phenotypes

10. How about if transgenic animals are made offsite   Using PHS funds?   Are you AAALAC accredited; is your collaborator? - Who owns the animals   Need an MOU?

11. What did we miss?