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Figure S1 A. B. * * * * * * * * * * * * *. Figure S2 A. B. Fold Increase OxPhos Genes.

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Presentation on theme: "Figure S1 A. B. * * * * * * * * * * * * *. Figure S2 A. B. Fold Increase OxPhos Genes."— Presentation transcript:

1 Figure S1 A. B. * * * * * * * * * * * * *

2 Figure S2 A. B. Fold Increase OxPhos Genes

3 Figure S3 Concentration (mmol/L) SEL-ResistantSEL-Sensitive B. Relative Luciferase Activity (ATP) C. SEL-ResistantSEL-Sensitive ECAR (mpH/min) or OCR (pmol/min) A. SEL-Resistant SEL-Sensitive

4 Figure S4 P < 0.0001 CI >1.0 CI <1.0

5 Figure S5 Dead Cells (%) A. OCR (pmoles/min) B. PGC1 α [Treatment : Mock] C.

6 Figure S6 2 1 A. mTOR1/2 Activity RTK Activation Tumor Suppression MAPK Activity Proliferation 2 1 B.

7 P-S6 (S235_S236) Relative Protein Levels D24 D29WM1799UCSD354 SKMEL5 MEL624 P-AKT (T308) Relative Protein Levels D24 D29WM1799UCSD354 SKMEL5 MEL624 Cl. Caspase 7 Relative Protein Levels D24 D29WM1799UCSD354 SKMEL5 MEL624 Figure S6 P-MAPK (T202_Y204) Relative Protein Levels D24 D29WM1799UCSD354 SKMEL5 MEL624 C.

8 Figure S6 D. MAPK & mTOR1/2 Activity Proliferation Apoptosis Tumor Suppression RTK Activation AKT Activity

9 R 2 =0.32 p=0.03 R 2 =0.54 p=0.002 Figure S7 A. B.

10 Figure S7 E. RLU WM3854 mRNA [Treatment : Mock] MEL624 C. mRNA [Treatment : Mock] WM3854 D.

11 Figure S8 DAPI MITF Merge MockSEL AZD8055SEL+8055 A.

12 Figure S8 RLU MEL624 B. * * * RLU MEL624 C. * * *

13 Figure S8 D. MEL624 Risc PGC1  MITF mTOR RAPTOR RICTOR RAPOR+RICTOR PGC1α MITF mTOR P-AKT(S473) P-4EBP1(S65) P-S6(S235/236) Raptor Rictor S6 AKT WM3854 Risc PGC1  MITF mTOR RAPTOR RICTOR RAPTOR+RICTOR PGC1α MITF mTOR P-AKT(S473) P-4EBP1(S65) P-S6(S235/236) Raptor Rictor S6 AKT

14 Relative Intensity Figure S9 A. Mock B. 2014 8055 Effect of mTORC1/2 Inhibition C. Mock SEL AZD8055 SEL+AZD8055 PGC1 α MITF GAPDH AZD2014 SEL+AZD2014

15 Figure S10 % Inhibition SEL (  M) A. % Inhibition B. SEL (  M) OCR (pmol/min/25,000 cells) Time (min) O F C.

16 P-MAPK D. Parental Clone R1 Clone R2 E. P-S6 ParentalClone R1Clone R2 Figure S10

17 Figure S11 A375A375-R1 Inhibition (%) A375-R2 Concentration (  M) A. B.C. Inhibition (%) Concentration (  M) D.E.F. WM35WM35-R1WM35-R2

18 Figure S11 G. A375 A375-R1 Mock SelAZD8055 Sel+8055

19 Figure S12 Patient samples mRNA [Prog : Pre] A.

20 Figure S12 59 Melanomas 166 Lung Cancers 172 Hematopoietic & Lymphoid Cancers Relative PGC1  Expression Cancers by Tissue Type 59 Melanomas B.

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