1. Microbial Genetics - DNA Transfer l Review of Information Transfer in Cell l Recombination l Transfer of DNA &Genetic Recombination in Bacteria –Conjugation –Transformation –Transduction

2. Microbial Genetics - DNA Transfer

3. l Recombination – process by which DNa is broken and repaired resulting in an exchange of DNA between two DNA molecules to form new combinations of genes l Eukaryotic - crossing over during meiosis l Prokaryotic - occurs when DNA is transfered from one cell to another (transfer from a doner to a recipient)

4. Microbial Genetics - DNA Transfer Genetic Recombination

5. Microbial Genetics - DNA Transfer Conjugation

6. Microbial Genetics - DNA Transfer Normal or Low Frequency Conjugation

7. Microbial Genetics - DNA Transfer High Frequency Conjugation

8. Transformation

9. Transduction

10. Microbial Genetics - Regulation l Three types of regulation –Constitutive - gene is always turned on –Repression - gene is normally turned on but can be turned off in response to a metabolic signal –Induction - gene is normally turned off but can be turned on in response to a metabolic signal

11. Microbial Genetics - Regulation l Types of Regulation of mRNA synthesis –Induction - lac operon –Repression - trp operon

12. Microbial Genetics - Regulation